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[[Image:3l9q.png|left|200px]]


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==Crystal structure of human polymerase alpha-primase p58 iron-sulfur cluster domain==
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<StructureSection load='3l9q' size='340' side='right'caption='[[3l9q]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3l9q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L9Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L9Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.698&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_3l9q|  PDB=3l9q  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l9q OCA], [https://pdbe.org/3l9q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l9q RCSB], [https://www.ebi.ac.uk/pdbsum/3l9q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l9q ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l9/3l9q_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3l9q ConSurf].
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== Publication Abstract from PubMed ==
DNA replication requires priming of DNA templates by enzymes known as primases. Although DNA primase structures are available from archaea and bacteria, the mechanism of DNA priming in higher eukaryotes remains poorly understood in large part due to the absence of the structure of the unique, highly conserved C-terminal regulatory domain of the large subunit (p58C). Here, we present the structure of this domain determined to 1.7-A resolution by X-ray crystallography. The p58C structure reveals a novel arrangement of an evolutionarily conserved 4Fe-4S cluster buried deeply within the protein core and is not similar to any known protein structure. Analysis of the binding of DNA to p58C by fluorescence anisotropy measurements revealed a strong preference for ss/dsDNA junction substrates. This approach was combined with site-directed mutagenesis to confirm that the binding of DNA occurs to a distinctively basic surface on p58C. A specific interaction of p58C with the C-terminal domain of the intermediate subunit of replication protein A (RPA32C) was identified and characterized by isothermal titration calorimetry and NMR. Restraints from NMR experiments were used to drive computational docking of the two domains and generate a model of the p58C-RPA32C complex. Together, our results explain functional defects in human DNA primase mutants and provide insights into primosome loading on RPA-coated ssDNA and regulation of primase activity.


===Crystal structure of human polymerase alpha-primase p58 iron-sulfur cluster domain===
Insights into eukaryotic DNA priming from the structure and functional interactions of the 4Fe-4S cluster domain of human DNA primase.,Vaithiyalingam S, Warren EM, Eichman BF, Chazin WJ Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13684-9. Epub 2010 Jul 19. PMID:20643958<ref>PMID:20643958</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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{{ABSTRACT_PUBMED_20643958}}
 
==About this Structure==
[[3l9q]] is a 2 chain structure of [[RNA polymerase]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L9Q OCA].


==See Also==
==See Also==
*[[RNA polymerase]]
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:020643958</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Chazin, W J.]]
[[Category: Large Structures]]
[[Category: Eichman, B F.]]
[[Category: Chazin WJ]]
[[Category: Vaithiyalingam, S.]]
[[Category: Eichman BF]]
[[Category: Dna replication]]
[[Category: Vaithiyalingam S]]
[[Category: Dna-binding]]
[[Category: Dna-directed rna polymerase]]
[[Category: Iron]]
[[Category: Iron-sulfur]]
[[Category: Iron-sulfur cluster]]
[[Category: Metal-binding]]
[[Category: Nucleotidyltransferase]]
[[Category: Phosphoprotein]]
[[Category: Pol alpha]]
[[Category: Polymerase]]
[[Category: Primase]]
[[Category: Primosome]]
[[Category: Transcription]]
[[Category: Transferase]]
Retrieved from "https://proteopedia.org/3l9q"