|
|
| (45 intermediate revisions by 5 users not shown) |
| Line 1: |
Line 1: |
| | <StructureSection load='' size='350' side='right' caption='E. coli GroEL (green)/GroES (magenta) complex with ADP, AlF3, Mg+2 and K+ ions (PDB entry [[1pcq]])' scene='44/445432/Cv/1'> |
| [[Image:1pcq.png|left|200px|thumb|Crystal Structure of Chaperonin, [[1pcq]]]] | | [[Image:1pcq.png|left|200px|thumb|Crystal Structure of Chaperonin, [[1pcq]]]] |
| {{STRUCTURE_1pcq| PDB=1pcq | SIZE=300| SCENE=Chaperonin/Groel_groes_comnplex/1 |right|CAPTION=GroEL/GroES complex, [[1pcq]] }}
| | '''Chaperonins''' (Cpn) are oligomeric proteins that mediate the folding of polypeptide chains. '''Group I CPN''' are found in bacteria, chloroplasts and mitochondria. For an introductory overview, see [http://en.wikipedia.org/wiki/Chaperonins Chaperonins in Wikipedia]. |
|
| |
|
| Chaperonin (CPN) are oligomeric proteins which mediate the folding of polypeptide chains. Group I CPNs are found in bacteria, chloroplasts and mitochondria. They include the most characterized GroEL/GroES complex from ''Escherichia coli'' and CPN60/CPN10 from ''Thermus thermophilus''. The larger subunit (GroEL, CPN60) contains 3 domains. The apical domain is the one which binds the substrate. Group II CPNs are found in eukaryotic cytosol and archaea. Thermosome is a CPN complex found in archaea. CCT is a CPN complex found in eukarya.
| | The most characterized Cpn are in the GroEL/GroES complex from ''Escherichia coli'' and Cpn60/Cpn10 from ''Thermus thermophilus''.<ref>PMID:18987317</ref> The larger subunit (GroEL, Cpn60) contains 3 domains: apical, intermediate and equatorial domain. The apical domain is the one which binds the polypeptide substrate. The equatorial domains binds the nucleotide. |
| | | *'''Group II Cpns''' are found during mitosis in eukaryotic cytosol and archaea. |
| {{TOC limit|limit=2}}
| | *'''Thermosome''' is a Cpn complex found in archaea.<ref>PMID:9546398</ref> |
| | *'''CCT''' or '''TRiC''' is a Cpn complex found in eukarya believed to be involved in uncontrolled proliferation.<ref>PMID:22503819</ref>, <ref>PMID:32297209</ref> |
| | *<scene name='44/445432/Cv/2'>E. coli GroEL/GroES complex</scene> (GroEL in green, GroES in magenta, PDB entry [[1pcq]]). |
| | *<scene name='44/445432/Cv/3'>GroEL/GroES complex with ADP, AlF3, Mg+2 and K+ ions</scene>. |
| | *<scene name='44/445432/Cv/5'>K+ ion coordination site</scene>. GroEL in cyan. |
| | *<scene name='44/445432/Cv/6'>AlF3 binding site</scene>. |
| | *<scene name='44/445432/Cv/7'>Mg+2 ion coordination site</scene>. |
| | *<scene name='44/445432/Cv/9'>ADP binding site</scene>. |
| | *<scene name='44/445432/Cv/10'>Whole binding site</scene>. |
| | *See also [[Chaperones]].<br /> |
| | *For GroEl in Hebrew see [[Sand box groel]]. |
|
| |
|
| == 3D Structures of Chaperonin == | | == 3D Structures of Chaperonin == |
| | [[Chaperonin 3D structures]] |
|
| |
|
| ''Updated November 2011''
| | == Files for 3D printer == |
| | | <i class="fas fa-cubes"></i> Asymmetric Chaperonin Complex GroEL/GroES by [[User:Marius Mihasan|Marius Mihasan]] [https://3dprint.nih.gov/discover/3dpx-017057 <i class="fas fa-download"></i>] |
| ===Group I=== | |
| | |
| ''Large subunit''
| |
| | |
| [[3m6c]] – MtGroEL1 apical domain – ''Mycobacterium tuberculosis''<BR />
| |
| [[1sjp]], [[3rtk]] – MtGroEL2 residues 42-539<BR />
| |
| [[3fbh]], [[2eu1]], [[1j4z]], [[1kpo]], [[1oel]], [[1grl]], [[2yey]] – EcGroEL (mutant) - ''Escherichia coli''<BR />
| |
| [[3e76]], [[2nwc]], [[1xck]], [[1ss8]] – EcGroEL<BR />
| |
| [[3c9v]], [[3cau]] – EcGroEL – Cryo EM<BR />
| |
| [[2c7e]], [[1gr5]] - EcGroEL (mutant) – Cryo EM<BR />
| |
| [[1kp8]] - EcGroEL (mutant) + ATP<BR />
| |
| [[1sx3]] - EcGroEL + ATP<BR />
| |
| [[1sx4]] - EcGroEL + ADP<BR />
| |
| [[1mnf]] – EcGroEL + polypeptide<BR />
| |
| [[2cgt]] – EcGroEL + capsid assbly protein GP31 – EM<BR />
| |
| [[1fy9]], [[1fya]], [[1kid]] – EcGroEL apical domain (mutant) <BR />
| |
| [[1srv]] – TtCPN60 apical domain - ''Thermus thermophilus''<BR />
| |
| [[3osx]] - CPN60 apical domain – ''Xenorhabdus nematophila''<br />
| |
| [[1iok]] – CPN60 – ''Paracoccus denitrificans''
| |
| | |
| ''Small subunit''
| |
| | |
| [[3nx6]] – GroES residues 40-134 (mutant) – ''Xanthomonas oryzae''<BR />
| |
| [[1wnr]] – TtCPN10 residues 1-94<BR /> | |
| [[1p3h]], [[1hx5]] – MtCPN10<BR />
| |
| [[1p82]], [[1p83]] - MtCPN10 residues 1-25 - NMR<BR />
| |
| [[1egs]] - GroES residues 19-27 - NMR
| |
| | |
| ''Large + small subunit''
| |
| | |
| [[1svt]], [[1pcq]] - EcGroEL + GroES<BR />
| |
| [[1pf9]], [[1aon]] - EcGroEL + GroES + ADP<BR />
| |
| [[2c7c]], [[2c7d]] - EcGroEL + GroES – Cryo EM<BR />
| |
| [[1gru]] - EcGroEL + GroES + ATP + ADP – Cryo EM<BR />
| |
| [[1we3]] – TtCPN60 + CPN10<BR />
| |
| [[1wf4]] - TtCPN60 + CPN10 + ADP
| |
| | |
| ===Group II===
| |
| | |
| [[3izh]], [[3izi]], [[3izj]], [[3izk]], [[3izl]], [[3izm]], [[3izn]], [[3los]], [[3iyf]] – MmCPN – ''Methanococcus maripaludis'' – EM<BR />
| |
| [[3kfb]], [[3kfe]], [[3kfk]] – MmCPN<BR />
| |
| [[1q2v]], [[1q3r]] – TkCPN α subunit (mutant) – ''Thermococcus'' KS-1<BR />
| |
| [[1q3q]] - TkCPN α subunit (mutant) + AMP-PNP<BR />
| |
| [[1q3s]] - TkCPN α subunit (mutant) + ADP
| |
| | |
| ''CCT''
| |
| | |
| [[2xsm]] – bCPN CCT – bovine<BR />
| |
| [[3p9d]], [[3p9e]] – CPN CCT - yeast<br />
| |
| [[3iyg]] - bCPN CCT – Cryo EM<BR />
| |
| [[3ktt]] - bCPN CCT β subunit – Cryo EM<BR />
| |
| [[1gml]], [[1gn1]] - CPN CCT γ subunit apical domain – mouse
| |
| | |
| ''Hsp33''
| |
| | |
| [[3m7m]], [[1hw7]] – EcCPN Hsp33 N-terminal<BR />
| |
| [[1xjh]] - EcCPN Hsp33 C terminal - NMR<BR />
| |
| [[1vq0]] - CPN Hsp33 – ''Thermotoga maritima''<BR />
| |
| [[1vzy]] - CPN Hsp33 (mutant) – ''Bacillus subtilis''
| |
| | |
| ''Thermosome''
| |
| | |
| [[1a6d]] - TaTherm α+β subunits – ''Thermoplasma acidophilum''<BR />
| |
| [[1a6e]] - TaTherm α+β subunits + ADP<BR />
| |
| [[1ass]], [[1asx]] - TaTherm α apical domain<BR />
| |
| [[1e0r]] – TaTherm β apical domain<br />
| |
| [[3ko1]] – Therm – ''Acidianus tengchongensis''<br />
| |
| [[3aq1]] – Therm – ''Methanococcoides burtonii''<br />
| |
| [[1lep]] – CPN-10 – ''Mycobacterium leprae''
| |
|
| |
|
| | </StructureSection> |
|
| |
|
| | == References == |
| | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |
| | [[Category:3D printer files]] |
