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[[Image:1gp7.gif|left|200px]]<br />
<applet load="1gp7" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1gp7, resolution 2.6&Aring;" />
'''ACIDIC PHOSPHOLIPASE A2 FROM VENOM OF OPHIOPHAGUS HANNAH'''<br />


==Overview==
==Acidic Phospholipase A2 from venom of Ophiophagus Hannah==
The crystal structure of an acidic phospholipase A(2) from Ophiophagus, hannah (king cobra) has been determined by molecular replacement at 2.6-A, resolution to a crystallographic R factor of 20.5% (R(free)=23.3%) with, reasonable stereochemistry. The venom enzyme contains an unusual, "pancreatic loop." The conformation of the loop is well defined and, different from those in pancreas PLA(2), showing its structural, variability. This analysis provides the first structure of a PLA(2)-type, cardiotoxin. The sites related to the cardiotoxic and myotoxic activities, are explored and the oligomer observed in the crystalline state is, described.
<StructureSection load='1gp7' size='340' side='right'caption='[[1gp7]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1gp7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Ophiophagus_hannah Ophiophagus hannah]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GP7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gp7 OCA], [https://pdbe.org/1gp7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gp7 RCSB], [https://www.ebi.ac.uk/pdbsum/1gp7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gp7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PA2A1_OPHHA PA2A1_OPHHA] Snake venom phospholipase A2 (PLA2) that may exhibit cardiotoxicity, myotoxicity, antiplatelet activity, and edema-inducing activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.<ref>PMID:9028866</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gp/1gp7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gp7 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of an acidic phospholipase A(2) from Ophiophagus hannah (king cobra) has been determined by molecular replacement at 2.6-A resolution to a crystallographic R factor of 20.5% (R(free)=23.3%) with reasonable stereochemistry. The venom enzyme contains an unusual "pancreatic loop." The conformation of the loop is well defined and different from those in pancreas PLA(2), showing its structural variability. This analysis provides the first structure of a PLA(2)-type cardiotoxin. The sites related to the cardiotoxic and myotoxic activities are explored and the oligomer observed in the crystalline state is described.


==About this Structure==
Structure of a cardiotoxic phospholipase A(2) from Ophiophagus hannah with the "pancreatic loop".,Zhang HL, Xu SJ, Wang QY, Song SY, Shu YY, Lin ZJ J Struct Biol. 2002 Jun;138(3):207-15. PMID:12217659<ref>PMID:12217659</ref>
1GP7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Ophiophagus_hannah Ophiophagus hannah]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4]]. Structure known Active Site: CAA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GP7 OCA]].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of a cardiotoxic phospholipase A(2) from Ophiophagus hannah with the "pancreatic loop"., Zhang HL, Xu SJ, Wang QY, Song SY, Shu YY, Lin ZJ, J Struct Biol. 2002 Jun;138(3):207-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12217659 12217659]
</div>
<div class="pdbe-citations 1gp7" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ophiophagus hannah]]
[[Category: Ophiophagus hannah]]
[[Category: Phospholipase A(2)]]
[[Category: Lin Z]]
[[Category: Single protein]]
[[Category: Zhang H]]
[[Category: Lin, Z.]]
[[Category: Zhang, H.]]
[[Category: CA]]
[[Category: calcium]]
[[Category: cardiotoxic activity]]
[[Category: crystal structure]]
[[Category: hydrolase]]
[[Category: king cobra]]
[[Category: lipid degradation]]
[[Category: myotoxic activity]]
[[Category: pancreatic loop]]
[[Category: phospholipase a2]]
[[Category: snake venom]]
 
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Retrieved from "https://proteopedia.org/1gp7"