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'''Unreleased structure'''


The entry 3uwl is ON HOLD  until Paper Publication
==Crystal structure of Enteroccocus faecalis thymidylate synthase (EfTS) in complex with 5-formyl tetrahydrofolate==
<StructureSection load='3uwl' size='340' side='right'caption='[[3uwl]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3uwl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UWL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FFO:N-[4-({[(6S)-2-AMINO-5-FORMYL-4-OXO-3,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)BENZOYL]-L-GLUTAMIC+ACID'>FFO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwl OCA], [https://pdbe.org/3uwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uwl RCSB], [https://www.ebi.ac.uk/pdbsum/3uwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uwl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TYSY_ENTFA TYSY_ENTFA] Provides the sole de novo source of dTMP for DNA biosynthesis.[HAMAP-Rule:MF_00008]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Drug resistance to therapeutic antibiotics poses a challenge to the identification of novel targets and drugs for the treatment of infectious diseases. Infections caused by Enterococcus faecalis are a major health problem. Thymidylate synthase (TS) from E. faecalis is a potential target for antibacterial therapy. The X-ray crystallographic structure of E. faecalis thymidylate synthase (EfTS), which was obtained as a native binary complex composed of EfTS and 5-formyltetrahydrofolate (5-FTHF), has been determined. The structure provides evidence that EfTS is a half-of-the-sites reactive enzyme, as 5-FTHF is bound to two of the four independent subunits present in the crystal asymmetric unit. 5-FTHF is a metabolite of the one-carbon transfer reaction catalysed by 5-formyltetrahydrofolate cyclo-ligase. Kinetic studies show that 5-FTHF is a weak inhibitor of EfTS, suggesting that the EfTS-5-FTHF complex may function as a source of folates and/or may regulate one-carbon metabolism. The structure represents the first example of endogenous 5-FTHF bound to a protein involved in folate metabolism.


Authors: Pozzi, C., Catalano, A., Cortesi, D., Luciani, R., Ferrari, S., Fritz, T., Costi, M.P., Mangani, S.
The structure of Enterococcus faecalis thymidylate synthase provides clues about folate bacterial metabolism.,Pozzi C, Ferrari S, Cortesi D, Luciani R, Stroud RM, Catalano A, Costi MP, Mangani S Acta Crystallogr D Biol Crystallogr. 2012 Sep;68(Pt 9):1232-41. doi:, 10.1107/S0907444912026236. Epub 2012 Aug 18. PMID:22948925<ref>PMID:22948925</ref>


Description: Crystal structure of Enteroccocus faecalis thymidylate synthase (EfTS) in complex with 5-formyl tetrahydrofolate
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3uwl" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Thymidylate synthase 3D structures|Thymidylate synthase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Enterococcus faecalis]]
[[Category: Large Structures]]
[[Category: Catalano A]]
[[Category: Cortesi D]]
[[Category: Costi MP]]
[[Category: Ferrari S]]
[[Category: Fritz T]]
[[Category: Luciani R]]
[[Category: Mangani S]]
[[Category: Pozzi C]]
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