3azv: Difference between revisions

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[[Image:3azv.jpg|left|200px]]


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==Crystal structure of the receptor binding domain==
The line below this paragraph, containing "STRUCTURE_3azv", creates the "Structure Box" on the page.
<StructureSection load='3azv' size='340' side='right'caption='[[3azv]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3azv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AZV FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_3azv|  PDB=3azv  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3azv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azv OCA], [https://pdbe.org/3azv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3azv RCSB], [https://www.ebi.ac.uk/pdbsum/3azv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3azv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9LBR1_CLOBO Q9LBR1_CLOBO]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Clostridium botulinum type D strain OFD05, which produces the D/C mosaic neurotoxin, was isolated from cattle killed by the recent botulism outbreak in Japan. The D/C mosaic neurotoxin is the most toxic of the botulinum neurotoxins (BoNT) characterized to date. Here, we determined the crystal structure of the receptor binding domain of BoNT from strain OFD05 in complex with 3'-sialyllactose at a resolution of 3.0A. In the structure, an electron density derived from the 3'-sialyllactose was confirmed at the cleft in the C-terminal subdomain. Alanine site-directed mutagenesis showed the significant contribution of the residues surrounding the cleft to ganglioside recognition. In addition, a loop adjoining the cleft also plays an important role in ganglioside recognition. In contrast, little effect was observed when the residues located around the surface previously identified as the protein receptor binding site in other BoNTs were substituted. The results of cell binding analysis of the mutants were significantly correlated with the ganglioside binding properties. Based on these observations, a cell binding mechanism of BoNT from strain OFD05 is proposed, which involves cooperative contribution of two ganglioside binding sites.


===Crystal structure of the receptor binding domain===
Structural and mutational analyses of the receptor binding domain of botulinum D/C mosaic neurotoxin: insight into the ganglioside binding mechanism.,Nuemket N, Tanaka Y, Tsukamoto K, Tsuji T, Nakamura K, Kozaki S, Yao M, Tanaka I Biochem Biophys Res Commun. 2011 Jul 29;411(2):433-9. Epub 2011 Jul 2. PMID:21749855<ref>PMID:21749855</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
The line below this paragraph, {{ABSTRACT_PUBMED_21749855}}, adds the Publication Abstract to the page
<div class="pdbe-citations 3azv" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 21749855 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_21749855}}
__TOC__
 
</StructureSection>
==About this Structure==
[[3azv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZV OCA].
 
==Reference==
<ref group="xtra">PMID:021749855</ref><references group="xtra"/>
[[Category: Clostridium botulinum]]
[[Category: Clostridium botulinum]]
[[Category: Kozaki, S.]]
[[Category: Large Structures]]
[[Category: Nakamura, K.]]
[[Category: Kozaki S]]
[[Category: Nuemket, N.]]
[[Category: Nakamura K]]
[[Category: Tanaka, I.]]
[[Category: Nuemket N]]
[[Category: Tanaka, Y.]]
[[Category: Tanaka I]]
[[Category: Tsuji, T.]]
[[Category: Tanaka Y]]
[[Category: Tsukamoto, K.]]
[[Category: Tsuji T]]
[[Category: Yao, M.]]
[[Category: Tsukamoto K]]
[[Category: Beta-sheet jellyroll]]
[[Category: Yao M]]
[[Category: Beta-trefoil fold]]
[[Category: Extra-cellular]]
[[Category: Ganglioside]]
[[Category: Receptor binding]]
[[Category: Toxin]]

Latest revision as of 08:50, 11 October 2023

Crystal structure of the receptor binding domain

3azv, resolution 3.10Å

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