Sandbox 206: Difference between revisions

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=== Introduction===

Yellow Fluorescent protein (YFP) is a variant of Green fluorescent protein (GFP). A lot of search was made of this protein in order to improve his fluorescence, his viability in different middle and others conditions<ref> Improved green fluorescence; Heim R., Cubitt A.B. and Tsien R.Y; Nature 373, 663 - 664 (23 February 1995) Pubmed : [http://www.ncbi.nlm.nih.gov/pubmed/7854443] DOI : [http://www.nature.com/nature/journal/v373/n6516/abs/373663b0.html] Disponible on [http://tsienlab.ucsd.edu/Publications/Heim%201995%20Nature%20-%20Improved%20GFP.PDF]</ref>.. This protein is very useful like a biomarker in a lot of biological applications. First, the GFP was mutated In YFP. But the YFP had a low fluorescence because of a slow maturation and it was very sensitive to experimental condition (pH, Cl-). To increase the brightness of the protein, maturation speed and other properties, five mutations were done to obtain Venus, a Yellow Fluorescent Protein less sensitive to experimental condition.

Yellow Fluorescent protein (YFP) is a variant of Green fluorescent protein (GFP). A lot of search was made of this protein in order to improve his fluorescence, his viability in different middle and others conditions<ref> Improved green fluorescence; Heim R., Cubitt A.B. and Tsien R.Y; Nature 373, 663 - 664 (23 February 1995) Pubmed : [http://www.ncbi.nlm.nih.gov/pubmed/7854443 7854443] DOI : [http://www.nature.com/nature/journal/v373/n6516/abs/373663b0.html 10.1038/373663b0] Disponible on [http://tsienlab.ucsd.edu/Publications/Heim%201995%20Nature%20-%20Improved%20GFP.PDF http://tsienlab.ucsd.edu/Publications/Heim%201995%20Nature%20-%20Improved%20GFP.PDF]</ref>. This protein is very useful like a biomarker in a lot of biological applications. First, the GFP was mutated In YFP. But the YFP had a low fluorescence because of a slow maturation and it was very sensitive to experimental condition (pH, Cl-)<ref>1H, 15N and 13C assignments of yellow fluorescent protein (YFP) Venus; Hsu ST, Behrens C, Cabrita LD, Dobson CM.; Biomol NMR Assign. 2009 Jun;3(1):67-72. Epub 2009 Jan 13 Pubmed : [http://www.ncbi.nlm.nih.gov/pubmed/19636949 19636949] DOI : [http://www.citeulike.org/article/3897162 doi:10.1007/s12104-009-9143-y] Disponible on [https://springerlink3.metapress.com/content/k45571u5l6341v44/resource-secured/?target=fulltext.pdf&sid=dfpkgoxtq2cck1regroonku4&sh=www.springerlink.com https://springerlink3.metapress.com]</ref>. To increase the brightness of the protein, maturation speed and other properties, five mutations were done to obtain Venus, a Yellow Fluorescent Protein less sensitive to experimental condition.

=== Presentation of the molecule ===

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 === Introduction===
-Yellow Fluorescent protein (YFP) is a variant of Green fluorescent protein (GFP). A lot of search was made of this protein in order to improve his fluorescence, his viability in different middle and others conditions<ref> Improved green fluorescence; Heim R., Cubitt A.B. and Tsien R.Y; Nature 373, 663 - 664 (23 February 1995) Pubmed : [http://www.ncbi.nlm.nih.gov/pubmed/7854443] DOI : [http://www.nature.com/nature/journal/v373/n6516/abs/373663b0.html] Disponible on [http://tsienlab.ucsd.edu/Publications/Heim%201995%20Nature%20-%20Improved%20GFP.PDF]</ref>.. This protein is very useful like a biomarker in a lot of biological applications. First, the GFP was mutated In YFP. But the YFP had a low fluorescence because of a slow maturation and it was very sensitive to experimental condition (pH, Cl-). To increase the brightness of the protein, maturation speed and other properties, five mutations were done to obtain Venus, a Yellow Fluorescent Protein less sensitive to experimental condition.
+Yellow Fluorescent protein (YFP) is a variant of Green fluorescent protein (GFP). A lot of search was made of this protein in order to improve his fluorescence, his viability in different middle and others conditions<ref> Improved green fluorescence; Heim R., Cubitt A.B. and Tsien R.Y; Nature 373, 663 - 664 (23 February 1995) Pubmed : [http://www.ncbi.nlm.nih.gov/pubmed/7854443 7854443] DOI : [http://www.nature.com/nature/journal/v373/n6516/abs/373663b0.html 10.1038/373663b0] Disponible on [http://tsienlab.ucsd.edu/Publications/Heim%201995%20Nature%20-%20Improved%20GFP.PDF http://tsienlab.ucsd.edu/Publications/Heim%201995%20Nature%20-%20Improved%20GFP.PDF]</ref>. This protein is very useful like a biomarker in a lot of biological applications. First, the GFP was mutated In YFP. But the YFP had a low fluorescence because of a slow maturation and it was very sensitive to experimental condition (pH, Cl-)<ref>1H, 15N and 13C assignments of yellow fluorescent protein (YFP) Venus; Hsu ST, Behrens C, Cabrita LD, Dobson CM.; Biomol NMR Assign. 2009 Jun;3(1):67-72. Epub 2009 Jan 13 Pubmed : [http://www.ncbi.nlm.nih.gov/pubmed/19636949 19636949] DOI : [http://www.citeulike.org/article/3897162 doi:10.1007/s12104-009-9143-y] Disponible on [https://springerlink3.metapress.com/content/k45571u5l6341v44/resource-secured/?target=fulltext.pdf&sid=dfpkgoxtq2cck1regroonku4&sh=www.springerlink.com https://springerlink3.metapress.com]</ref>. To increase the brightness of the protein, maturation speed and other properties, five mutations were done to obtain Venus, a Yellow Fluorescent Protein less sensitive to experimental condition.
 === Presentation of the molecule ===