3t6s: Difference between revisions
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< | ==Crystal structure of CerJ from Streptomyces tendae in Complex with CoA== | ||
<StructureSection load='3t6s' size='340' side='right'caption='[[3t6s]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3t6s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_tendae Streptomyces tendae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T6S FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t6s OCA], [https://pdbe.org/3t6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t6s RCSB], [https://www.ebi.ac.uk/pdbsum/3t6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t6s ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/H2L2M1_STRTE H2L2M1_STRTE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ketosynthases produce the carbon backbones of a vast number of biologically active polyketides by catalyzing Claisen condensations of activated acyl and malonyl building blocks. Here we report that a ketosynthase homolog from Streptomyces tendae, CerJ, unexpectedly forms malonyl esters during the biosynthesis of cervimycin, a glycoside antibiotic against methicillin-resistant Staphylococcus aureus (MRSA). Deletion of cerJ yielded a substantially more active cervimycin variant lacking the malonyl side chain, and in vitro biotransformations revealed that CerJ is capable of transferring malonyl, methylmalonyl and dimethylmalonyl units onto the glycoside. According to phylogenetic analyses and elucidation of the crystal structure, CerJ is functionally and structurally positioned between the ketosynthase catalyzing Claisen condensations and acyl-ACP shuttles, and it features a noncanonical catalytic triad. Site-directed mutagenesis and structures of CerJ in complex with substrates not only allowed us to establish a model for the reaction mechanism but also provided insights into the evolution of this important subclass of the thiolase superfamily. | |||
A ketosynthase homolog uses malonyl units to form esters in cervimycin biosynthesis.,Bretschneider T, Zocher G, Unger M, Scherlach K, Stehle T, Hertweck C Nat Chem Biol. 2011 Dec 18. doi: 10.1038/nchembio.746. PMID:22179067<ref>PMID:22179067</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3t6s" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | [[Category: Large Structures]] | ||
== | |||
< | |||
[[Category: Streptomyces tendae]] | [[Category: Streptomyces tendae]] | ||
[[Category: Bretschneider | [[Category: Bretschneider T]] | ||
[[Category: Hertweck | [[Category: Hertweck C]] | ||
[[Category: Stehle | [[Category: Stehle T]] | ||
[[Category: Zocher | [[Category: Zocher G]] | ||
Latest revision as of 02:26, 21 November 2024
Crystal structure of CerJ from Streptomyces tendae in Complex with CoA
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