4a5n: Difference between revisions

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[[Image:4a5n.png|left|200px]]


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==Redoxregulator HypR in its reduced form==
The line below this paragraph, containing "STRUCTURE_4a5n", creates the "Structure Box" on the page.
<StructureSection load='4a5n' size='340' side='right'caption='[[4a5n]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[4a5n]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A5N FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
{{STRUCTURE_4a5n|  PDB=4a5n  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a5n OCA], [https://pdbe.org/4a5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a5n RCSB], [https://www.ebi.ac.uk/pdbsum/4a5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a5n ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/YYBR_BACSU YYBR_BACSU]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacillus subtilis encodes redox-sensing MarR-type regulators of the OhrR and DUF24-families that sense organic hydroperoxides, diamide, quinones or aldehydes via thiol-based redox-switches. In this article, we characterize the novel redox-sensing MarR/DUF24-family regulator HypR (YybR) that is activated by disulphide stress caused by diamide and NaOCl in B. subtilis. HypR controls positively a flavin oxidoreductase HypO that confers protection against NaOCl stress. The conserved N-terminal Cys14 residue of HypR has a lower pK(a) of 6.36 and is essential for activation of hypO transcription by disulphide stress. HypR resembles a 2-Cys-type regulator that is activated by Cys14-Cys49' intersubunit disulphide formation. The crystal structures of reduced and oxidized HypR proteins were resolved revealing structural changes of HypR upon oxidation. In reduced HypR a hydrogen-bonding network stabilizes the reactive Cys14 thiolate that is 8-9 A apart from Cys49'. HypR oxidation breaks these H-bonds, reorients the monomers and moves the major groove recognition alpha4 and alpha4' helices approximately 4 A towards each other. This is the first crystal structure of a redox-sensing MarR/DUF24 family protein in bacteria that is activated by NaOCl stress. Since hypochloric acid is released by activated macrophages, related HypR-like regulators could function to protect pathogens against the host immune defense.


===Redoxregulator HypR in its reduced form===
Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR.,Palm GJ, Khanh Chi B, Waack P, Gronau K, Becher D, Albrecht D, Hinrichs W, Read RJ, Antelmann H Nucleic Acids Res. 2012 May 1;40(9):4178-92. Epub 2012 Jan 11. PMID:22238377<ref>PMID:22238377</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==About this Structure==
</div>
[[4a5n]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A5N OCA].
<div class="pdbe-citations 4a5n" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Hinrichs, W.]]
[[Category: Large Structures]]
[[Category: Palm, G J.]]
[[Category: Hinrichs W]]
[[Category: Read, R J.]]
[[Category: Palm GJ]]
[[Category: Waack, P.]]
[[Category: Read RJ]]
[[Category: Activator]]
[[Category: Waack P]]
[[Category: Dna binding]]
[[Category: Marr-like]]
[[Category: Transcription]]

Latest revision as of 11:19, 20 December 2023

Redoxregulator HypR in its reduced form

4a5n, resolution 1.81Å

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