3v6t: Difference between revisions

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[[Image:3v6t.jpg|left|200px]]


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==Crystal structure of the DNA-bound dHax3, a TAL effector, at 1.85 angstrom==
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<StructureSection load='3v6t' size='340' side='right'caption='[[3v6t]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3v6t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas Xanthomonas]. The December 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''TAL Effectors''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_12 10.2210/rcsb_pdb/mom_2014_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V6T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v6t OCA], [https://pdbe.org/3v6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v6t RCSB], [https://www.ebi.ac.uk/pdbsum/3v6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v6t ProSAT]</span></td></tr>
{{STRUCTURE_3v6t|  PDB=3v6t  |  SCENE=  }}
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
TAL effectors, secreted by phytopathogenic bacteria, recognize host DNA sequences through a central domain of tandem repeats. Each repeat comprises 33 to 35 conserved amino acids and targets a specific base pair using two hypervariable residues (known as RVD) at positions 12 and 13. Here, we report the crystal structures of a 11.5-repeat TAL effector in both DNA-free and DNA-bound states. Each TAL repeat comprises two helices connected by a short RVD-containing loop. The 11.5 repeats form a right-handed, super-helical structure that tracks along the sense strand of DNA duplex, with RVDs contacting the major groove. The 12th residue stabilizes the RVD loop, whereas the 13th residue makes a base-specific contact. Understanding DNA recognition by TAL effectors may facilitate rational design of DNA-binding proteins with biotechnological applications.


===Crystal structure of the DNA-bound dHax3, a TAL effector, at 1.85 angstrom===
Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors.,Deng D, Yan C, Pan X, Mahfouz M, Wang J, Zhu JK, Shi Y, Yan N Science. 2012 Jan 5. PMID:22223738<ref>PMID:22223738</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3v6t" style="background-color:#fffaf0;"></div>


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==See Also==
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*[[TAL effector|TAL effector]]
(as it appears on PubMed at http://www.pubmed.gov), where 22223738 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_22223738}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3v6t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthomonas Xanthomonas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V6T OCA].
[[Category: RCSB PDB Molecule of the Month]]
 
[[Category: TAL Effectors]]
==Reference==
<ref group="xtra">PMID:022223738</ref><references group="xtra"/>
[[Category: Xanthomonas]]
[[Category: Xanthomonas]]
[[Category: Deng, D.]]
[[Category: Deng D]]
[[Category: Pan, X J.]]
[[Category: Pan XJ]]
[[Category: Shi, Y G.]]
[[Category: Shi YG]]
[[Category: Wang, J W.]]
[[Category: Wang JW]]
[[Category: Yan, C Y.]]
[[Category: Yan CY]]
[[Category: Yan, N.]]
[[Category: Yan N]]
[[Category: 11 tandem repeat]]
[[Category: Dna binding protein-dna complex]]
[[Category: Dna specific binding protein]]
Retrieved from "https://proteopedia.org/3v6t"