3axf: Difference between revisions

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[[Image:3axf.jpg|left|200px]]


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==Perrhenate binding to A11C/R153C ModA mutant==
The line below this paragraph, containing "STRUCTURE_3axf", creates the "Structure Box" on the page.
<StructureSection load='3axf' size='340' side='right'caption='[[3axf]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3axf]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AXF FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=REO:PERRHENATE'>REO</scene></td></tr>
{{STRUCTURE_3axf|  PDB=3axf  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3axf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3axf OCA], [https://pdbe.org/3axf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3axf RCSB], [https://www.ebi.ac.uk/pdbsum/3axf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3axf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MODA_ECOLI MODA_ECOLI] Involved in the transport of molybdenum into the cell. Binds molybdate with high specificity and affinity.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Radiolabeled biomolecules are routinely used for clinical diagnostics. (99m)Tc is the most commonly used radioactive tracer in radiopharmaceuticals. (188)Re and (186)Re are also commonly used as radioactive tracers in medicine. However, currently available methods for radiolabeling are lengthy and involve several steps in bioconjugation processes. In this work we present a strategy to engineer proteins that may selectively recognize the perrhenate (ReO(4) (-)) ion as a new way to label proteins. We found that a molybdate (MoO(4) (2-))-binding protein (ModA) from Escherichia coli can bind perrhenate with high affinity. Using fluorescence and isothermal titration calorimetry measurements, we determined the dissociation constant of ModA for ReO(4) (-) to be 541 nM and we solved a crystal structure of ModA with a bound ReO(4) (-). On the basis of the structure we created a mutant protein containing a disulfide linkage, which exhibited increased affinity for perrhenate (K (d) = 104 nM). High-resolution crystal structures of ModA (1.7 A) and A11C/R153C mutant (2.0 A) were solved with bound perrhenate. Both structures show that a perrhenate ion occupies the molybdate binding site using the same amino acid residues that are involved in molybdate binding. The overall structure of the perrhenate-bound ModA is unchanged compared with that of the molybdate-bound form. In the mutant protein, the bound perrhenate is further stabilized by the engineered disulfide bond.


===Perrhenate binding to A11C/R153C ModA mutant===
Binding of ReO(4) (-) with an engineered MoO (4) (2-)-binding protein: towards a new approach in radiopharmaceutical applications.,Aryal BP, Brugarolas P, He C J Biol Inorg Chem. 2012 Jan;17(1):97-106. Epub 2011 Aug 23. PMID:21861186<ref>PMID:21861186</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3axf" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_21861186}}, adds the Publication Abstract to the page
*[[ABC transporter 3D structures|ABC transporter 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 21861186 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_21861186}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Escherichia coli K-12]]
[[3axf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AXF OCA].
[[Category: Large Structures]]
 
[[Category: Aryal BP]]
==Reference==
[[Category: Brugarolas P]]
<ref group="xtra">PMID:021861186</ref><references group="xtra"/>
[[Category: He C]]
[[Category: Escherichia coli]]
[[Category: Aryal, B P.]]
[[Category: Brugarolas, P.]]
[[Category: He, C.]]
[[Category: Metal binding protein]]
[[Category: Molybdate binding protein]]

Latest revision as of 08:54, 13 August 2026

Perrhenate binding to A11C/R153C ModA mutant

3axf, resolution 2.00Å

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