3t5b: Difference between revisions

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[[Image:3t5b.png|left|200px]]


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==Crystal structure of N-terminal domain of FACL13 from Mycobacterium tuberculosis==
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<StructureSection load='3t5b' size='340' side='right'caption='[[3t5b]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3t5b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T5B FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5b OCA], [https://pdbe.org/3t5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t5b RCSB], [https://www.ebi.ac.uk/pdbsum/3t5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5b ProSAT]</span></td></tr>
{{STRUCTURE_3t5b|  PDB=3t5b  |  SCENE=  }}
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Activation of fatty acids as acyl-adenylates by fatty acyl-AMP ligases (FAALs) in Mycobacterium tuberculosis is a variant of a classical theme that involves formation of acyl-CoA (coenzyme A) by fatty acyl-CoA ligases (FACLs). Here, we show that FAALs and FACLs possess similar structural fold and substrate specificity determinants, and the key difference is the absence of a unique insertion sequence in FACL13 structure. A systematic analysis shows a conserved hydrophobic anchorage of the insertion motif across several FAALs. Strikingly, mutagenesis of two phenylalanine residues, which are part of the anchorage, to alanine converts FAAL32 to FACL32. This insertion-based in silico analysis suggests the presence of FAAL homologues in several other non-mycobacterial genomes including eukaryotes. The work presented here establishes an elegant mechanism wherein an insertion sequence drives the functional divergence of FAALs from canonical FACLs.


===Crystal structure of N-terminal domain of FACL13 from Mycobacterium tuberculosis===
Molecular Basis of the Functional Divergence of Fatty Acyl-AMP Ligase Biosynthetic Enzymes of Mycobacterium tuberculosis.,Goyal A, Verma P, Anandhakrishnan M, Gokhale RS, Sankaranarayanan R J Mol Biol. 2011 Dec 21. PMID:22206988<ref>PMID:22206988</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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(as it appears on PubMed at http://www.pubmed.gov), where 22206988 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_22206988}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3t5b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5B OCA].
[[Category: Mycobacterium tuberculosis H37Rv]]
 
[[Category: Goyal A]]
==Reference==
[[Category: Sankaranarayanan R]]
<ref group="xtra">PMID:022206988</ref><references group="xtra"/>
[[Category: Mycobacterium tuberculosis]]
[[Category: Goyal, A.]]
[[Category: Sankaranarayanan, R.]]
[[Category: Acetyl-coa synthetase like fold]]
[[Category: Amp-binding]]
[[Category: Ligase]]
Retrieved from "https://proteopedia.org/3t5b"