3qwb: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:3qwb.jpg|left|200px]]


<!--
==Crystal structure of Saccharomyces cerevisiae Zeta-crystallin-like quinone oxidoreductase Zta1 complexed with NADPH==
The line below this paragraph, containing "STRUCTURE_3qwb", creates the "Structure Box" on the page.
<StructureSection load='3qwb' size='340' side='right'caption='[[3qwb]], [[Resolution|resolution]] 1.59&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3qwb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QWB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QWB FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.59&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
{{STRUCTURE_3qwb|  PDB=3qwb  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qwb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qwb OCA], [https://pdbe.org/3qwb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qwb RCSB], [https://www.ebi.ac.uk/pdbsum/3qwb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qwb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/QOR_YEAST QOR_YEAST]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Quinone oxidoreductase (QOR EC1.6.5.5) catalyzes the reduction of quinone to hydroxyquinone using NADPH as a cofactor. Here we present the crystal structure of the zeta-crystallin-like QOR Zta1 from Saccharomycescerevisiae in apo-form at 2.00 A and complexed with NADPH at 1.59 A resolution. Zta1 forms a homodimer, with each subunit containing a catalytic and a cofactor-binding domain. Upon NADPH binding to the interdomain cleft, the two domains shift towards each other, producing a better fit for NADPH, and tightening substrate binding. Computational simulation combined with site-directed mutagenesis and enzymatic activity analysis defined a potential quinone-binding site that determines the stringent substrate specificity. Moreover, multiple-sequence alignment and kinetics assays implied that a single-residue change from Arg in lower organisms to Gly in vertebrates possibly resulted in elevation of enzymatic activity of zeta-crystallin-like QORs throughout evolution.


===Crystal structure of Saccharomyces cerevisiae Zeta-crystallin-like quinone oxidoreductase Zta1 complexed with NADPH===
Structural insights into the cofactor-assisted substrate recognition of yeast quinone oxidoreductase Zta1.,Guo PC, Ma XX, Bao ZZ, Ma JD, Chen Y, Zhou CZ J Struct Biol. 2011 Oct;176(1):112-8. Epub 2011 Jul 26. PMID:21820057<ref>PMID:21820057</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<!--
</div>
The line below this paragraph, {{ABSTRACT_PUBMED_21820057}}, adds the Publication Abstract to the page
<div class="pdbe-citations 3qwb" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 21820057 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_21820057}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3qwb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QWB OCA].
[[Category: Saccharomyces cerevisiae S288C]]
 
[[Category: Bao ZZ]]
==Reference==
[[Category: Chen YX]]
<ref group="xtra">PMID:021820057</ref><references group="xtra"/>
[[Category: Guo PC]]
[[Category: NADPH:quinone reductase]]
[[Category: Ma XX]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Zhou CZ]]
[[Category: Bao, Z Z.]]
[[Category: Chen, Y X.]]
[[Category: Guo, P C.]]
[[Category: Ma, X X.]]
[[Category: Zhou, C Z.]]
[[Category: Cytoplasm and nucleus]]
[[Category: Nadph]]
[[Category: Oxidoreductase]]
[[Category: Quinone oxidoreductase]]
[[Category: Rossmann fold]]

Latest revision as of 17:15, 1 November 2023

Crystal structure of Saccharomyces cerevisiae Zeta-crystallin-like quinone oxidoreductase Zta1 complexed with NADPH

3qwb, resolution 1.59Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=3qwb&oldid=3936573"