|
|
| (14 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| [[Image:2q2k.jpg|left|200px]]<br /><applet load="2q2k" size="350" color="white" frame="true" align="right" spinBox="true"
| |
| caption="2q2k, resolution 3.00Å" />
| |
| '''Structure of nucleic-acid binding protein'''<br />
| |
|
| |
|
| ==Overview== | | ==Structure of nucleic-acid binding protein== |
| The stable inheritance of genetic material depends on accurate DNA, partition. Plasmids serve as tractable model systems to study DNA, segregation because they require only a DNA centromere, a, centromere-binding protein and a force-generating ATPase. The centromeres, of partition (par) systems typically consist of a tandem arrangement of, direct repeats. The best-characterized par system contains a, centromere-binding protein called ParR and an ATPase called ParM. In the, first step of segregation, multiple ParR proteins interact with the, centromere repeats to form a large nucleoprotein complex of unknown, structure called the segrosome, which binds ParM filaments. pSK41 ParR, binds a centromere consisting of multiple 20-base-pair (bp) tandem repeats, to mediate both transcription autoregulation and segregation. Here we, report the structure of the pSK41 segrosome revealed in the crystal, structure of a ParR-DNA complex. In the crystals, the 20-mer tandem, repeats stack pseudo-continuously to generate the full-length centromere, with the ribbon-helix-helix (RHH) fold of ParR binding successive DNA, repeats as dimer-of-dimers. Remarkably, the dimer-of-dimers assemble in a, continuous protein super-helical array, wrapping the DNA about its, positive convex surface to form a large segrosome with an open, solenoid-shaped structure, suggesting a mechanism for ParM capture and, subsequent plasmid segregation.
| | <StructureSection load='2q2k' size='340' side='right'caption='[[2q2k]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[2q2k]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q2K FirstGlance]. <br> |
| 2Q2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=EPE:'>EPE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q2K OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5IU:5-IODO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>5IU</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr> |
| ==Reference==
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q2k OCA], [https://pdbe.org/2q2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q2k RCSB], [https://www.ebi.ac.uk/pdbsum/2q2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q2k ProSAT]</span></td></tr> |
| Segrosome structure revealed by a complex of ParR with centromere DNA., Schumacher MA, Glover TC, Brzoska AJ, Jensen SO, Dunham TD, Skurray RA, Firth N, Nature. 2007 Dec 20;450(7173):1268-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18097417 18097417]
| | </table> |
| [[Category: Single protein]] | | == Function == |
| | [https://www.uniprot.org/uniprot/O87365_STAAU O87365_STAAU] |
| | __TOC__ |
| | </StructureSection> |
| | [[Category: Large Structures]] |
| [[Category: Staphylococcus aureus]] | | [[Category: Staphylococcus aureus]] |
| [[Category: Firth, N.]] | | [[Category: Firth N]] |
| [[Category: Glover, T.]] | | [[Category: Glover T]] |
| [[Category: Schumacher, M.A.]] | | [[Category: Schumacher MA]] |
| [[Category: EPE]]
| |
| [[Category: dna binding protein/dna complex]]
| |
| [[Category: parb]]
| |
| [[Category: partition]]
| |
| [[Category: protein-dna]]
| |
| [[Category: segregation]]
| |
| | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 13 08:14:24 2008''
| |
