2lkd: Difference between revisions

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[[Image:2lkd.jpg|left|200px]]


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==IF2-G2 GDP complex==
The line below this paragraph, containing "STRUCTURE_2lkd", creates the "Structure Box" on the page.
<StructureSection load='2lkd' size='340' side='right'caption='[[2lkd]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2lkd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LKD FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
{{STRUCTURE_2lkd|  PDB=2lkd  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lkd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lkd OCA], [https://pdbe.org/2lkd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lkd RCSB], [https://www.ebi.ac.uk/pdbsum/2lkd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lkd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IF2_GEOSE IF2_GEOSE] One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial translation initiation factor IF2 promotes ribosomal subunit association, recruitment, and binding of fMet-tRNA to the ribosomal P-site and initiation dipeptide formation. Here, we present the solution structures of GDP-bound and apo-IF2-G2 of Bacillus stearothermophilus and provide evidence that this isolated domain binds the 50 S ribosomal subunit and hydrolyzes GTP. Differences between the free and GDP-bound structures of IF2-G2 suggest that domain reorganization within the G2-G3-C1 regions underlies the different structural requirements of IF2 during the initiation process. However, these structural signals are unlikely forwarded from IF2-G2 to the C-terminal fMet-tRNA binding domain (IF2-C2) because the connected IF2-C1 and IF2-C2 modules show completely independent mobility, indicating that the bacterial interdomain connector lacks the rigidity that was found in the archaeal IF2 homolog aIF5B.


===IF2-G2 GDP complex===
Structural Dynamics of Bacterial Translation Initiation Factor IF2.,Wienk H, Tishchenko E, Belardinelli R, Tomaselli S, Dongre R, Spurio R, Folkers GE, Gualerzi CO, Boelens R J Biol Chem. 2012 Mar 30;287(14):10922-32. Epub 2012 Feb 3. PMID:22308033<ref>PMID:22308033</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==About this Structure==
</div>
[[2lkd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKD OCA].
<div class="pdbe-citations 2lkd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
[[Category: Belardinelli, R.]]
[[Category: Large Structures]]
[[Category: Boelens, R.]]
[[Category: Belardinelli R]]
[[Category: Dongre, R.]]
[[Category: Boelens R]]
[[Category: Folkers, G E.]]
[[Category: Dongre R]]
[[Category: Gualerzi, C O.]]
[[Category: Folkers GE]]
[[Category: Spurio, R.]]
[[Category: Gualerzi CO]]
[[Category: Tishchenko, E.]]
[[Category: Spurio R]]
[[Category: Tomaselli, S.]]
[[Category: Tishchenko E]]
[[Category: Wienk, H.]]
[[Category: Tomaselli S]]
[[Category: Translation]]
[[Category: Wienk H]]
Retrieved from "https://proteopedia.org/2lkd"