4aij: Difference between revisions
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New page: '''Unreleased structure''' The entry 4aij is ON HOLD until Paper Publication Authors: Quade, N., Mendonca, C., Herbst, K., Heroven, A.K., Heinz, D.W., Dersch, P. Description: Crystal s... |
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==Crystal structure of RovA from Yersinia in complex with a rovA promoter fragment== | |||
<StructureSection load='4aij' size='340' side='right'caption='[[4aij]], [[Resolution|resolution]] 2.05Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4aij]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis Yersinia pseudotuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AIJ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aij OCA], [https://pdbe.org/4aij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aij RCSB], [https://www.ebi.ac.uk/pdbsum/4aij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aij ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/SLYA_YERPY SLYA_YERPY] Transcription regulator that can specifically activate or repress expression of target genes.[HAMAP-Rule:MF_01819] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Pathogens often rely on thermosensing to adjust virulence gene expression. In yersiniae, important virulence-associated traits are under the control of the master regulator RovA which uses an in-built thermosensor to control its activity. Thermal upshifts encountered upon host entry induce conformational changes of the RovA dimer that attenuate DNA-binding and render the protein more susceptible to proteolysis. Here, we report the crystal structure of RovA in the free and DNA-bound form and provide evidence that thermo-induced loss of RovA activity is mainly promoted by a thermosensing loop in the dimerization domain and residues in the adjacent C-terminal helix. These determinants allow partial unfolding of the regulator upon an upshift to 37 degrees C. This structural distortion is transmitted to the flexible DNA-binding domain of RovA. RovA mainly contacts the DNA backbone in a low affinity-binding mode which allows the immediate release of RovA from its operator sites. We also show that SlyA, a close homologue of RovA from Salmonella with a very similar structure, is not a thermosensor and remains active and stable at 37 degrees C. Strikingly, changes in only three amino acids, reflecting evolutionary replacements in SlyA, result in a complete loss of the thermosensing properties of RovA and prevent degradation. In conclusion, only minor alterations can transform a thermotolerant regulator into a thermosensor that allows adjustment of virulence and fitness determinants to their thermal environment. | |||
Structural basis for intrinsic thermosensing by the master virulence regulator RovA of Yersinia.,Quade N, Mendonca C, Herbst K, Heroven AK, Ritter C, Heinz DW, Dersch P J Biol Chem. 2012 Aug 30. PMID:22936808<ref>PMID:22936808</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4aij" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Yersinia pseudotuberculosis]] | |||
[[Category: Dersch P]] | |||
[[Category: Heinz DW]] | |||
[[Category: Herbst K]] | |||
[[Category: Heroven AK]] | |||
[[Category: Mendonca C]] | |||
[[Category: Quade N]] | |||
