3k3w: Difference between revisions

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[[Image:3k3w.png|left|200px]]


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==Thermostable Penicillin G acylase from Alcaligenes faecalis in orthorhombic form==
The line below this paragraph, containing "STRUCTURE_3k3w", creates the "Structure Box" on the page.
<StructureSection load='3k3w' size='340' side='right'caption='[[3k3w]], [[Resolution|resolution]] 3.31&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3k3w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K3W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K3W FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.31&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
{{STRUCTURE_3k3w|  PDB=3k3w  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k3w OCA], [https://pdbe.org/3k3w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k3w RCSB], [https://www.ebi.ac.uk/pdbsum/3k3w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k3w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O34142_ALCFA O34142_ALCFA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k3/3k3w_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k3w ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The enzyme penicillin G acylase (EC 3.5.1.11) catalyzes amide-bond cleavage in benzylpenicillin (penicillin G) to yield 6-aminopenicillanic acid, an intermediate chemical used in the production of semisynthetic penicillins. A thermostable penicillin G acylase from Alcaligenes faecalis (AfPGA) has been crystallized using the hanging-drop vapour-diffusion method in two different space groups: C222(1), with unit-cell parameters a = 72.9, b = 86.0, c = 260.2 , and P4(1)2(1)2, with unit-cell parameters a = b = 85.6, c = 298.8 . Data were collected at 293 and the structure was determined using the molecular-replacement method. Like other penicillin acylases, AfPGA belongs to the N-terminal nucleophilic hydrolase superfamily, has undergone post-translational processing and has a serine as the N-terminal residue of the beta-chain. A disulfide bridge has been identified in the structure that was not found in the other two known penicillin G cylase structures. The presence of the disulfide bridge is perceived to be one factor that confers higher stability to this enzyme.


===Thermostable Penicillin G acylase from Alcaligenes faecalis in orthorhombic form===
Crystallization and X-ray structure analysis of a thermostable penicillin G acylase from Alcaligenes faecalis.,Varshney NK, Kumar RS, Ignatova Z, Prabhune A, Pundle A, Dodson E, Suresh CG Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Mar 1;68(Pt 3):273-7. Epub, 2012 Feb 15. PMID:22442220<ref>PMID:22442220</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3k3w" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_14622260}}, adds the Publication Abstract to the page
*[[Penicillin acylase|Penicillin acylase]]
(as it appears on PubMed at http://www.pubmed.gov), where 14622260 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_14622260}}
__TOC__
 
</StructureSection>
==About this Structure==
[[3k3w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K3W OCA].
 
==Reference==
<ref group="xtra">PMID:014622260</ref><references group="xtra"/>
[[Category: Alcaligenes faecalis]]
[[Category: Alcaligenes faecalis]]
[[Category: Penicillin amidase]]
[[Category: Large Structures]]
[[Category: Dodson, E.]]
[[Category: Dodson E]]
[[Category: Ignatova, Z.]]
[[Category: Ignatova Z]]
[[Category: Kumar, R S.]]
[[Category: Kumar RS]]
[[Category: Suresh, C G.]]
[[Category: Suresh CG]]
[[Category: Varshney, N K.]]
[[Category: Varshney NK]]
[[Category: Hydrolase]]
[[Category: Penicillin g acylase]]
Retrieved from "https://proteopedia.org/3k3w"