3u9g: Difference between revisions

Latest revision as of 08:38, 20 March 2024

Crystal structure of the Zinc finger antiviral protein

Structural highlights

3u9g is a 1 chain structure with sequence from Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.801Å
Ligands:	ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ZCCHV_RAT Antiviral protein which inhibits the replication of viruses by recruiting the cellular RNA degradation machineries to degrade the viral mRNAs. Binds to a ZAP-responsive element (ZRE) present in the target viral mRNA, recruits cellular poly(A)-specific ribonuclease PARN to remove the poly(A) tail, and the 3'-5' exoribonuclease complex exosome to degrade the RNA body from the 3'-end. It also recruits the decapping complex DCP1-DCP2 through RNA helicase p72 (DDX17) to remove the cap structure of the viral mRNA to initiate its degradation from the 5'-end. Its target viruses belong to families which include retroviridae: human immunodeficiency virus type 1 (HIV-1) and moloney and murine leukemia virus (MoMLV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), togaviridae: sindbis virus (SINV) and Ross river virus (RRV). Specifically targets the multiply spliced but not unspliced or singly spliced HIV-1 mRNAs for degradation.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

References

↑ Gao G, Guo X, Goff SP. Inhibition of retroviral RNA production by ZAP, a CCCH-type zinc finger protein. Science. 2002 Sep 6;297(5587):1703-6. PMID:12215647 doi:https://dx.doi.org/10.1126/science.1074276
↑ Bick MJ, Carroll JW, Gao G, Goff SP, Rice CM, MacDonald MR. Expression of the zinc-finger antiviral protein inhibits alphavirus replication. J Virol. 2003 Nov;77(21):11555-62. PMID:14557641
↑ Muller S, Moller P, Bick MJ, Wurr S, Becker S, Gunther S, Kummerer BM. Inhibition of filovirus replication by the zinc finger antiviral protein. J Virol. 2007 Mar;81(5):2391-400. Epub 2006 Dec 20. PMID:17182693 doi:https://dx.doi.org/10.1128/JVI.01601-06
↑ Guo X, Ma J, Sun J, Gao G. The zinc-finger antiviral protein recruits the RNA processing exosome to degrade the target mRNA. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):151-6. Epub 2006 Dec 21. PMID:17185417 doi:https://dx.doi.org/10.1073/pnas.0607063104
↑ MacDonald MR, Machlin ES, Albin OR, Levy DE. The zinc finger antiviral protein acts synergistically with an interferon-induced factor for maximal activity against alphaviruses. J Virol. 2007 Dec;81(24):13509-18. Epub 2007 Oct 10. PMID:17928353 doi:https://dx.doi.org/10.1128/JVI.00402-07
↑ Kerns JA, Emerman M, Malik HS. Positive selection and increased antiviral activity associated with the PARP-containing isoform of human zinc-finger antiviral protein. PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1371/journal.pgen.0040021. PMID:18225958 doi:https://dx.doi.org/10.1371/journal.pgen.0040021
↑ Zhu Y, Chen G, Lv F, Wang X, Ji X, Xu Y, Sun J, Wu L, Zheng YT, Gao G. Zinc-finger antiviral protein inhibits HIV-1 infection by selectively targeting multiply spliced viral mRNAs for degradation. Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15834-9. doi:, 10.1073/pnas.1101676108. Epub 2011 Aug 29. PMID:21876179 doi:https://dx.doi.org/10.1073/pnas.1101676108

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Gao G, Guo X, Goff SP. Inhibition of retroviral RNA production by ZAP, a CCCH-type zinc finger protein. Science. 2002 Sep 6;297(5587):1703-6. PMID:12215647 doi:https://dx.doi.org/10.1126/science.1074276

[2] Bick MJ, Carroll JW, Gao G, Goff SP, Rice CM, MacDonald MR. Expression of the zinc-finger antiviral protein inhibits alphavirus replication. J Virol. 2003 Nov;77(21):11555-62. PMID:14557641

[3] Muller S, Moller P, Bick MJ, Wurr S, Becker S, Gunther S, Kummerer BM. Inhibition of filovirus replication by the zinc finger antiviral protein. J Virol. 2007 Mar;81(5):2391-400. Epub 2006 Dec 20. PMID:17182693 doi:https://dx.doi.org/10.1128/JVI.01601-06

[4] Guo X, Ma J, Sun J, Gao G. The zinc-finger antiviral protein recruits the RNA processing exosome to degrade the target mRNA. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):151-6. Epub 2006 Dec 21. PMID:17185417 doi:https://dx.doi.org/10.1073/pnas.0607063104

[5] MacDonald MR, Machlin ES, Albin OR, Levy DE. The zinc finger antiviral protein acts synergistically with an interferon-induced factor for maximal activity against alphaviruses. J Virol. 2007 Dec;81(24):13509-18. Epub 2007 Oct 10. PMID:17928353 doi:https://dx.doi.org/10.1128/JVI.00402-07

[6] Kerns JA, Emerman M, Malik HS. Positive selection and increased antiviral activity associated with the PARP-containing isoform of human zinc-finger antiviral protein. PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1371/journal.pgen.0040021. PMID:18225958 doi:https://dx.doi.org/10.1371/journal.pgen.0040021

[7] Zhu Y, Chen G, Lv F, Wang X, Ji X, Xu Y, Sun J, Wu L, Zheng YT, Gao G. Zinc-finger antiviral protein inhibits HIV-1 infection by selectively targeting multiply spliced viral mRNAs for degradation. Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15834-9. doi:, 10.1073/pnas.1101676108. Epub 2011 Aug 29. PMID:21876179 doi:https://dx.doi.org/10.1073/pnas.1101676108

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@@ Line 1: / Line 1: @@
-[[Image:3u9g.jpg|left|200px]]
-<!--
+==Crystal structure of the Zinc finger antiviral protein==
-The line below this paragraph, containing "STRUCTURE_3u9g", creates the "Structure Box" on the page.
+<StructureSection load='3u9g' size='340' side='right'caption='[[3u9g]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3u9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U9G FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.801&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_3u9g|  PDB=3u9g  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u9g OCA], [https://pdbe.org/3u9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u9g RCSB], [https://www.ebi.ac.uk/pdbsum/3u9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u9g ProSAT]</span></td></tr>
+</table>
-===Crystal structure of the Zinc finger antiviral protein===
+== Function ==
+[https://www.uniprot.org/uniprot/ZCCHV_RAT ZCCHV_RAT] Antiviral protein which inhibits the replication of viruses by recruiting the cellular RNA degradation machineries to degrade the viral mRNAs. Binds to a ZAP-responsive element (ZRE) present in the target viral mRNA, recruits cellular poly(A)-specific ribonuclease PARN to remove the poly(A) tail, and the 3'-5' exoribonuclease complex exosome to degrade the RNA body from the 3'-end. It also recruits the decapping complex DCP1-DCP2 through RNA helicase p72 (DDX17) to remove the cap structure of the viral mRNA to initiate its degradation from the 5'-end. Its target viruses belong to families which include retroviridae: human immunodeficiency virus type 1 (HIV-1) and moloney and murine leukemia virus (MoMLV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), togaviridae: sindbis virus (SINV) and Ross river virus (RRV). Specifically targets the multiply spliced but not unspliced or singly spliced HIV-1 mRNAs for degradation.<ref>PMID:12215647</ref> <ref>PMID:14557641</ref> <ref>PMID:17182693</ref> <ref>PMID:17185417</ref> <ref>PMID:17928353</ref> <ref>PMID:18225958</ref> <ref>PMID:21876179</ref>
+== References ==
-==About this Structure==
+<references/>
-[[3u9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U9G OCA].
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Rattus norvegicus]]
-[[Category: Chen, S.]]
+[[Category: Chen S]]
-[[Category: Gao, G.]]
+[[Category: Gao G]]
-[[Category: Liu, Y.]]
+[[Category: Liu Y]]
-[[Category: Sun, J.]]
+[[Category: Sun J]]
-[[Category: Wang, X.]]
+[[Category: Wang X]]
-[[Category: Xu, Y.]]
+[[Category: Xu Y]]
-[[Category: Zhang, K.]]
+[[Category: Zhang K]]
-[[Category: Antiviral protein]]
-[[Category: Zinc finger protein]]

3u9g: Difference between revisions

Latest revision as of 08:38, 20 March 2024

Crystal structure of the Zinc finger antiviral protein

Structural highlights

Function

References

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