2xrp: Difference between revisions
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< | ==Human Doublecortin N-DC Repeat (1MJD) and Mammalian Tubulin (1JFF and 3HKE) Docked into the 8-Angstrom Cryo-EM Map of Doublecortin- Stabilised Microtubules== | ||
<SX load='2xrp' size='340' side='right' viewer='molstar' caption='[[2xrp]], [[Resolution|resolution]] 8.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2xrp]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XRP FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xrp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xrp OCA], [https://pdbe.org/2xrp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xrp RCSB], [https://www.ebi.ac.uk/pdbsum/2xrp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xrp ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Microtubule-associated proteins (MAPs) are essential for regulating and organizing cellular microtubules (MTs). However, our mechanistic understanding of MAP function is limited by a lack of detailed structural information. Using cryo-electron microscopy and single particle algorithms, we solved the 8 A structure of doublecortin (DCX)-stabilized MTs. Because of DCX's unusual ability to specifically nucleate and stabilize 13-protofilament MTs, our reconstruction provides unprecedented insight into the structure of MTs with an in vivo architecture, and in the absence of a stabilizing drug. DCX specifically recognizes the corner of four tubulin dimers, a binding mode ideally suited to stabilizing both lateral and longitudinal lattice contacts. A striking consequence of this is that DCX does not bind the MT seam. DCX binding on the MT surface indirectly stabilizes conserved tubulin-tubulin lateral contacts in the MT lumen, operating independently of the nucleotide bound to tubulin. DCX's exquisite binding selectivity uncovers important insights into regulation of cellular MTs. | |||
Template-free 13-protofilament microtubule-MAP assembly visualized at 8 A resolution.,Fourniol FJ, Sindelar CV, Amigues B, Clare DK, Thomas G, Perderiset M, Francis F, Houdusse A, Moores CA J Cell Biol. 2010 Nov 1;191(3):463-70. Epub 2010 Oct 25. PMID:20974813<ref>PMID:20974813</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2xrp" style="background-color:#fffaf0;"></div> | |||
== | |||
==See Also== | ==See Also== | ||
*[[Tubulin]] | *[[Tubulin 3D Structures|Tubulin 3D Structures]] | ||
*[[Tubulin tyrosine ligase 3D structures|Tubulin tyrosine ligase 3D structures]] | |||
== | == References == | ||
< | <references/> | ||
__TOC__ | |||
</SX> | |||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Amigues | [[Category: Amigues B]] | ||
[[Category: Clare | [[Category: Clare DK]] | ||
[[Category: Fourniol | [[Category: Fourniol FJ]] | ||
[[Category: Francis | [[Category: Francis F]] | ||
[[Category: Houdusse | [[Category: Houdusse A]] | ||
[[Category: Moores | [[Category: Moores CA]] | ||
[[Category: Perderiset | [[Category: Perderiset M]] | ||
[[Category: Sindelar | [[Category: Sindelar CV]] | ||
[[Category: Thomas | [[Category: Thomas G]] | ||
Latest revision as of 10:29, 9 May 2024
Human Doublecortin N-DC Repeat (1MJD) and Mammalian Tubulin (1JFF and 3HKE) Docked into the 8-Angstrom Cryo-EM Map of Doublecortin- Stabilised Microtubules
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