3a0e: Difference between revisions

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[[Image:3a0e.png|left|200px]]


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==Crystal Structure of Polygonatum cyrtonema lectin (PCL) complexed with dimannoside==
The line below this paragraph, containing "STRUCTURE_3a0e", creates the "Structure Box" on the page.
<StructureSection load='3a0e' size='340' side='right'caption='[[3a0e]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3a0e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Polygonatum_cyrtonema Polygonatum cyrtonema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A0E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A0E FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=PRD_900112:3alpha-alpha-mannobiose'>PRD_900112</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_3a0e|  PDB=3a0e  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a0e OCA], [https://pdbe.org/3a0e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a0e RCSB], [https://www.ebi.ac.uk/pdbsum/3a0e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a0e ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8L568_9ASPA Q8L568_9ASPA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a0/3a0e_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a0e ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Polygonatum cyrtonema lectin (PCL) is a novel anti-HIV mannose-binding lectin from Galanthus nivalis agglutinin (GNA)-related lectin family. Crystal structures of ligand-free PCL and its complexes with monomannoside and alpha1-3 dimannoside have been determined. The ligand-free PCL is dimeric, with both subunits adopt the beta-prism II fold. PCL subunit binds mannose using a potential bivalent mode instead of the usual trivalent mode, in which carbohydrate-binding site (CBS) I and CBS III adopt the conserved mannose-binding motif of QXDXNXVXY (X is one of any amino acid residues) as observed in other structurally characterized GNA-related lectins, while CBS II adopts a modified motif with residues Gln58 and Asp60, which are critical for mannose-binding, substituted by His58 and Asn60, respectively. As a result, CBS II is unfit for mannose-binding. In the mannoside complexes, ligand-bindings only occur at CBS I which provides the specificity for alpha1-3 dimannoside. CBS II and CBS III are cooperatively occupied by a well-ordered sulfate ion, through which the individual dimers are cross-linked to form a unique super-structure of 3(2) helical lattice. Surveying the sequences of GNA-related lectins revealed that the modified binding motif of CBS II is widely distributed in the Liliaceae family as an intrinsic structural element. There is evidence that other GNA-related lectins will also adopt the similar super-structure as PCL. Thus PCL structure, unique in ligand-binding mode, may represent a novel type of structure of GNA-related lectins. Comparative analyses indicated that the dimer-based super-structure may play a primary role in the anti-HIV property of PCL.


===Crystal Structure of Polygonatum cyrtonema lectin (PCL) complexed with dimannoside===
Crystal structures of a novel anti-HIV mannose-binding lectin from Polygonatum cyrtonema Hua with unique ligand-binding property and super-structure.,Ding J, Bao J, Zhu D, Zhang Y, Wang DC J Struct Biol. 2010 Sep;171(3):309-17. Epub 2010 May 28. PMID:20546901<ref>PMID:20546901</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3a0e" style="background-color:#fffaf0;"></div>


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==See Also==
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*[[Mannose-binding protein|Mannose-binding protein]]
(as it appears on PubMed at http://www.pubmed.gov), where 20546901 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_20546901}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3a0e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Polygonatum_cyrtonema Polygonatum cyrtonema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A0E OCA].
 
==Reference==
<ref group="xtra">PMID:020546901</ref><references group="xtra"/>
[[Category: Polygonatum cyrtonema]]
[[Category: Polygonatum cyrtonema]]
[[Category: Ding, J.]]
[[Category: Ding J]]
[[Category: Wang, D C.]]
[[Category: Wang DC]]
[[Category: Beta-prism ii]]
[[Category: Lectin]]
[[Category: Sugar binding protein]]
Retrieved from "https://proteopedia.org/3a0e"