3u44: Difference between revisions

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[[Image:3u44.jpg|left|200px]]


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==Crystal structure of AddAB-DNA complex==
The line below this paragraph, containing "STRUCTURE_3u44", creates the "Structure Box" on the page.
<StructureSection load='3u44' size='340' side='right'caption='[[3u44]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3u44]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U44 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U44 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.201&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u44 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u44 OCA], [https://pdbe.org/3u44 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u44 RCSB], [https://www.ebi.ac.uk/pdbsum/3u44 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u44 ProSAT]</span></td></tr>
{{STRUCTURE_3u44|  PDB=3u44  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/ADDA_BACSU ADDA_BACSU] An essential component of the DNA double-stranded break repair machinery, the heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the B.subtilis chi site (5'-AGCGG-3') which transforms the enzyme from a helicase which degrades both DNA strands to one with only 5' -> 3' exonuclease activity. This generates a double-stranded DNA with a protruding 3'-terminated single-stranded tail suitable for the initiation of homologous recombination (chi fragment). The AddA nuclease domain in particular is required for chi fragment generation; this subunit has 3' -> 5' nuclease and helicase activity. RecA thread formation during DNA double-strand break repair requires RecJ or AddAB.<ref>PMID:8387145</ref> <ref>PMID:10756102</ref> <ref>PMID:17570399</ref>
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== Publication Abstract from PubMed ==
In bacterial cells, processing of double-stranded DNA breaks for repair by homologous recombination is dependent upon the recombination hotspot sequence Chi and is catalysed by either an AddAB- or RecBCD-type helicase-nuclease. Here, we report the crystal structure of AddAB bound to DNA. The structure allows identification of a putative Chi-recognition site in an inactivated helicase domain of the AddB subunit. By generating mutant protein complexes that do not respond to Chi, we show that residues responsible for Chi recognition are located in positions equivalent to the signature motifs of a conventional helicase. Comparison with the related RecBCD complex, which recognizes a different Chi sequence, provides further insight into the structural basis for sequence-specific ssDNA recognition. The structure suggests a simple mechanism for DNA break processing, explains how AddAB and RecBCD can accomplish the same overall reaction with different sets of functional modules and reveals details of the role of an Fe-S cluster in protein stability and DNA binding.


===Crystal structure of AddAB-DNA complex===
Insights into Chi recognition from the structure of an AddAB-type helicase-nuclease complex.,Saikrishnan K, Yeeles JT, Gilhooly NS, Krajewski WW, Dillingham MS, Wigley DB EMBO J. 2012 Feb 3. doi: 10.1038/emboj.2012.9. PMID:22307084<ref>PMID:22307084</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3u44" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
{{ABSTRACT_PUBMED_22307084}}
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</StructureSection>
==About this Structure==
[[3u44]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U44 OCA].
 
==Reference==
<ref group="xtra">PMID:022307084</ref><references group="xtra"/>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Krajewski, W.]]
[[Category: Large Structures]]
[[Category: Saikrishnan, K.]]
[[Category: Krajewski W]]
[[Category: Wigley, D.]]
[[Category: Saikrishnan K]]
[[Category: Dna repair]]
[[Category: Wigley D]]
[[Category: Helicase]]
[[Category: Hydrolase-dna complex]]
[[Category: Nuclease]]
[[Category: Protein-dna complex]]

Latest revision as of 10:21, 13 August 2026

Crystal structure of AddAB-DNA complex

3u44, resolution 3.20Å

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