3rgp: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,615 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,615 edits
No edit summary
 
(9 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:3rgp.png|left|200px]]


<!--
==Structural and Kinetic Analysis of the Beef liver Catalase complexed with Nitric Oxide==
The line below this paragraph, containing "STRUCTURE_3rgp", creates the "Structure Box" on the page.
<StructureSection load='3rgp' size='340' side='right'caption='[[3rgp]], [[Resolution|resolution]] 1.88&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3rgp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RGP FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.88&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO:NITRIC+OXIDE'>NO</scene></td></tr>
{{STRUCTURE_3rgp|  PDB=3rgp  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rgp OCA], [https://pdbe.org/3rgp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rgp RCSB], [https://www.ebi.ac.uk/pdbsum/3rgp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rgp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CATA_BOVIN CATA_BOVIN] Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We present the structures of bovine catalase in its native form and complexed with ammonia and nitric oxide, obtained by X-ray crystallography. Using the NO generator 1-(N,N-diethylamino)diazen-1-ium-1,2-diolate, we were able to generate sufficiently high NO concentrations within the catalase crystals that substantial occupation was observed despite a high dissociation rate. Nitric oxide seems to be slightly bent from the heme normal that may indicate some iron(II) character in the formally ferric catalase. Microspectrophotometric investigations inline with the synchrotron X-ray beam reveal photoreduction of the central heme iron. In the cases of the native and ammonia-complexed catalase, reduction is accompanied by a relaxation phase. This is likely not the case for the catalase NO complex. The kinetics of binding of NO to catalase were investigated using NO photolyzed from N,N'-bis(carboxymethyl)-N,N'-dinitroso-p-phenylenediamine using an assay that combines catalase with myoglobin binding kinetics. The off rate is 1.5 s(-1). Implications for catalase function are discussed.


===Structural and Kinetic Analysis of the Beef liver Catalase complexed with Nitric Oxide===
Interaction of Nitric Oxide with Catalase: Structural and Kinetic Analysis.,Purwar N, McGarry JM, Kostera J, Pacheco AA, Schmidt M Biochemistry. 2011 May 6. PMID:21524057<ref>PMID:21524057</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<!--
</div>
The line below this paragraph, {{ABSTRACT_PUBMED_21524057}}, adds the Publication Abstract to the page
<div class="pdbe-citations 3rgp" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 21524057 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_21524057}}
 
==About this Structure==
[[3rgp]] is a 4 chain structure of [[Catalase]] with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGP OCA].


==See Also==
==See Also==
*[[Catalase|Catalase]]
*[[Catalase 3D structures|Catalase 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:021524057</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Catalase]]
[[Category: Large Structures]]
[[Category: Purwar, N.]]
[[Category: Purwar N]]
[[Category: Schmidt, M]]
[[Category: Schmidt M]]
[[Category: 6th coordination site as a binding pocket]]
[[Category: Binding of nitric oxide]]
[[Category: Heme protein]]
[[Category: Nitrosylation]]
[[Category: Oxidoreducatase]]
[[Category: Oxidoreductase]]
Retrieved from "https://proteopedia.org/3rgp"