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[[Image:1k0x.jpg|left|200px]]<br /><applet load="1k0x" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1k0x" />
'''Solution Structure of Melanoma Inhibitory Activity Protein'''<br />


==Overview==
==Solution Structure of Melanoma Inhibitory Activity Protein==
Melanoma inhibitory activity (MIA) is a small secreted protein that is, implicated in cartilage cell maintenance and melanoma metastasis. It is, representative of a recently discovered family of proteins that contain a, Src Homologous 3 (SH3) subdomain. While SH3 domains are normally found in, intracellular proteins and mediate protein-protein interactions via, recognition of polyproline helices, MIA is single-domain extracellular, protein, and it probably binds to a different class of ligands. Here we, report the assignments, solution structure, and dynamics of human MIA, determined by heteronuclear NMR methods. The structures were calculated in, a semi-automated manner without manual assignment of NOE crosspeaks, and, have a backbone rmsd of 0.38 A over the ordered regions of the protein., The structure consists of an SH3-like subdomain with N- and C-terminal, extensions of approximately 20 amino acids each that together form a novel, fold. The rmsd between the solution structure and our recently reported, crystal structure is 0.86 A over the ordered regions of the backbone, and, the main differences are localized to the most dynamic regions of the, protein. The similarity between the NMR and crystal structures supports, the use of automated NOE assignments and ambiguous restraints to, accelerate the calculation of NMR structures.
<StructureSection load='1k0x' size='340' side='right'caption='[[1k0x]]' scene=''>
 
== Structural highlights ==
==Disease==
<table><tr><td colspan='2'>[[1k0x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K0X FirstGlance]. <br>
Known disease associated with this structure: Myocardial infarction, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=611082 611082]]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0x OCA], [https://pdbe.org/1k0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k0x RCSB], [https://www.ebi.ac.uk/pdbsum/1k0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0x ProSAT]</span></td></tr>
==About this Structure==
</table>
1K0X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0X OCA].  
== Function ==
 
[https://www.uniprot.org/uniprot/MIA_HUMAN MIA_HUMAN] Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
==Reference==
== Evolutionary Conservation ==
Solution structure and dynamics of melanoma inhibitory activity protein., Lougheed JC, Domaille PJ, Handel TM, J Biomol NMR. 2002 Mar;22(3):211-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11991352 11991352]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/1k0x_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0x ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Domaille, P.J.]]
[[Category: Domaille PJ]]
[[Category: Handel, T.M.]]
[[Category: Handel TM]]
[[Category: Lougheed, J.C.]]
[[Category: Lougheed JC]]
[[Category: sh3 subdomain]]
 
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Retrieved from "https://proteopedia.org/1k0x"