3vcy: Difference between revisions

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[[Image:3vcy.png|left|200px]]


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==Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.==
The line below this paragraph, containing "STRUCTURE_3vcy", creates the "Structure Box" on the page.
<StructureSection load='3vcy' size='340' side='right'caption='[[3vcy]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3vcy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aliivibrio_fischeri_MJ11 Aliivibrio fischeri MJ11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VCY FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.925&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FFQ:[(1R)-1-HYDROXYPROPYL]PHOSPHONIC+ACID'>FFQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
{{STRUCTURE_3vcy|  PDB=3vcy  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vcy OCA], [https://pdbe.org/3vcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vcy RCSB], [https://www.ebi.ac.uk/pdbsum/3vcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vcy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MURA_ALIFM MURA_ALIFM] Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.[HAMAP-Rule:MF_00111]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The development of new antibiotics is necessitated by the rapid development of resistance to current therapies. UDP-N-acetylglucosamine enolpyruvyl transferase (MurA), which catalyzes the first committed step of bacterial peptidoglycan biosynthesis, is a prime candidate for therapeutic intervention. MurA is the target of the antibiotic fosfomycin, a natural product produced by Streptomyces. Despite possessing a high degree of sequence conservation with MurA enzymes from fosfomycin-susceptible organisms, recent microbiological studies suggest that MurA from Vibrio fischeri (VfiMurA) may confer fosfomycin resistance via a mechanism that is not yet understood. The crystal structure of VfiMurA in a ternary complex with the substrate UDP-N-acetylglucosamine (UNAG) and fosfomycin has been solved to a resolution of 1.93 A. Fosfomycin is known to inhibit MurA by covalently binding to a highly conserved cysteine in the active site of the enzyme. A comparison of the title structure with the structure of fosfomycin-susceptible Haemophilus influenzae MurA (PDB entry 2rl2) revealed strikingly similar conformations of the mobile substrate-binding loop and clear electron density for a fosfomycin-cysteine adduct. Based on these results, there are no distinguishing sequence/structural features in VfiMurA that would translate to a diminished sensitivity to fosfomycin. However, VfiMurA is a robust crystallizer and shares high sequence identity with many clinically relevant bacterial pathogens. Thus, it would serve as an ideal system for use in the structure-guided optimization of new antibacterial agents.


===Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.===
Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase) from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.,Bensen DC, Rodriguez S, Nix J, Cunningham ML, Tari LW Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Apr 1;68(Pt 4):382-5. Epub, 2012 Mar 27. PMID:22505403<ref>PMID:22505403</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3vcy" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_22505403}}, adds the Publication Abstract to the page
*[[Enoylpyruvate transferase 3D structures|Enoylpyruvate transferase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 22505403 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_22505403}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Aliivibrio fischeri MJ11]]
[[3vcy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aliivibrio_fischeri Aliivibrio fischeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA].
[[Category: Large Structures]]
 
[[Category: Bensen DC]]
==Reference==
[[Category: Cunningham ML]]
<ref group="xtra">PMID:022505403</ref><references group="xtra"/>
[[Category: Nix J]]
[[Category: Aliivibrio fischeri]]
[[Category: Rodriguez S]]
[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]
[[Category: Tari LW]]
[[Category: Bensen, D C.]]
[[Category: Cunningham, M L.]]
[[Category: Nix, J.]]
[[Category: Rodriguez, S.]]
[[Category: Tari, L W.]]
[[Category: Amino sugar and nucleotide sugar metabolism]]
[[Category: Cytosol]]
[[Category: Fosfomycin]]
[[Category: Mura]]
[[Category: Peptidoglycan]]
[[Category: Peptidoglycan biosynthesis]]
[[Category: Transferase-antibiotic complex]]

Latest revision as of 09:47, 30 October 2024

Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.

3vcy, resolution 1.93Å

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