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[[Image:1p4u.jpg|left|200px]]<br /><applet load="1p4u" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1p4u, resolution 2.20&Aring;" />
'''CRYSTAL STRUCTURE OF GGA3 GAE DOMAIN IN COMPLEX WITH RABAPTIN-5 PEPTIDE'''<br />


==Overview==
==CRYSTAL STRUCTURE OF GGA3 GAE DOMAIN IN COMPLEX WITH RABAPTIN-5 PEPTIDE==
Adaptor proteins load transmembrane protein cargo into transport vesicles, and serve as nexuses for the formation of large multiprotein complexes on, the nascent vesicles. The gamma-adaptin ear (GAE) domains of the AP-1, adaptor protein complex and the GGA adaptor proteins recruit accessory, proteins to these multiprotein complexes by binding to a hydrophobic, motif. We determined the structure of the GAE domain of human GGA3 in, complex with a peptide based on the DFGPLV sequence of the accessory, protein Rabaptin-5 and refined it at a resolution of 2.2 A. The leucine, and valine residues of the peptide are partly buried in two contiguous, shallow, hydrophobic depressions. The anchoring phenylalanine is buried in, a deep pocket formed by the aliphatic portions of two conserved arginine, residues, along with an alanine and a proline, illustrating the unusual, function of a cluster of basic residues in binding a hydrophobic motif.
<StructureSection load='1p4u' size='340' side='right'caption='[[1p4u]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1p4u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P4U FirstGlance]. <br>
1P4U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P4U OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p4u OCA], [https://pdbe.org/1p4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p4u RCSB], [https://www.ebi.ac.uk/pdbsum/1p4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p4u ProSAT]</span></td></tr>
==Reference==
</table>
Recognition of accessory protein motifs by the gamma-adaptin ear domain of GGA3., Miller GJ, Mattera R, Bonifacino JS, Hurley JH, Nat Struct Biol. 2003 Aug;10(8):599-606. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12858162 12858162]
== Function ==
[https://www.uniprot.org/uniprot/GGA3_HUMAN GGA3_HUMAN] Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.<ref>PMID:11301005</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p4/1p4u_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p4u ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Bonifacino, J.S.]]
[[Category: Bonifacino JS]]
[[Category: Hurley, J.H.]]
[[Category: Hurley JH]]
[[Category: Mattera, R.]]
[[Category: Mattera R]]
[[Category: Miller, G.J.]]
[[Category: Miller GJ]]
[[Category: protein transport]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:38:49 2008''

Latest revision as of 08:05, 14 February 2024

CRYSTAL STRUCTURE OF GGA3 GAE DOMAIN IN COMPLEX WITH RABAPTIN-5 PEPTIDE

1p4u, resolution 2.20Å

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