3vhf: Difference between revisions

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[[Image:3vhf.jpg|left|200px]]


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==plant thaumatin I at pH 8.0==
The line below this paragraph, containing "STRUCTURE_3vhf", creates the "Structure Box" on the page.
<StructureSection load='3vhf' size='340' side='right'caption='[[3vhf]], [[Resolution|resolution]] 1.39&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3vhf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VHF FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.39&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
{{STRUCTURE_3vhf|  PDB=3vhf  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vhf OCA], [https://pdbe.org/3vhf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vhf RCSB], [https://www.ebi.ac.uk/pdbsum/3vhf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vhf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/THM1_THADA THM1_THADA] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Thaumatin, an intensely sweet-tasting plant protein, elicits a sweet taste at 50 nM. Although the sweetness remains when thaumatin is heated at 80 degrees C for 4h under acid conditions, it rapidly declines when heating at a pH above 6.5. To clarify the structural difference at high pH, the atomic structure of a recombinant thaumatin I at pH 8.0 was determined at a resolution of 1.0A. Comparison to the crystal structure of thaumatin at pH 7.3 and 7.0 revealed the root-mean square deviation value of a Calpha atom to be substantially greater in the large disulfide-rich region of domain II, especially residues 154-164, suggesting that a loop region in domain II to be affected by solvent conditions. Furthermore, B-factors of Lys137, Lys163, and Lys187 were significantly affected by pH change, suggesting that a striking increase in the mobility of these lysine residues, which could facilitate a reaction with a free sulfhydryl residue produced via the beta-elimination of disulfide bonds by heating at a pH above 7.0. The increase in mobility of lysine residues as well as a loop region in domain II might play an important role in the heat-induced aggregation of thaumatin above pH 7.0.


===plant thaumatin I at pH 8.0===
Atomic structure of the sweet-tasting protein thaumatin I at pH 8.0 reveals the large disulfide-rich region in domain II to be sensitive to a pH change.,Masuda T, Ohta K, Mikami B, Kitabatake N, Tani F Biochem Biophys Res Commun. 2012 Mar 2;419(1):72-6. Epub 2012 Feb 3. PMID:22326916<ref>PMID:22326916</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3vhf" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 22326916 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_22326916}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3vhf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VHF OCA].
 
==Reference==
<ref group="xtra">PMID:022326916</ref><references group="xtra"/>
[[Category: Thaumatococcus daniellii]]
[[Category: Thaumatococcus daniellii]]
[[Category: Kitabatake, N.]]
[[Category: Kitabatake N]]
[[Category: Masuda, T.]]
[[Category: Masuda T]]
[[Category: Mikami, B.]]
[[Category: Mikami B]]
[[Category: Tani, F.]]
[[Category: Tani F]]
[[Category: Aril]]
[[Category: Mainly beta]]
[[Category: Plant protein]]
[[Category: Sweet receptor]]
[[Category: Sweet-tasting protein]]
[[Category: Taste protein]]
[[Category: Thaumatin family]]
Retrieved from "https://proteopedia.org/3vhf"