4dbl: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
 
(5 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:4dbl.png|left|200px]]


<!--
==Crystal structure of E159Q mutant of BtuCDF==
The line below this paragraph, containing "STRUCTURE_4dbl", creates the "Structure Box" on the page.
<StructureSection load='4dbl' size='340' side='right'caption='[[4dbl]], [[Resolution|resolution]] 3.49&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[4dbl]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DBL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DBL FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.493&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_4dbl|  PDB=4dbl  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dbl OCA], [https://pdbe.org/4dbl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dbl RCSB], [https://www.ebi.ac.uk/pdbsum/4dbl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dbl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BTUC_ECOLI BTUC_ECOLI] Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Involved in the translocation of the substrate across the membrane.[HAMAP-Rule:MF_01004]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BtuCD is an ABC transporter catalyzing the uptake of vitamin B(12) across the Escherichia coli inner membrane. A previously reported X-ray structure of BtuCD in complex with the periplasmic vitamin B(12)-binding protein BtuF revealed asymmetry of the transmembrane BtuC subunits. The functional relevance of this asymmetry has remained uncertain. Here we report the X-ray structure of a catalytically impaired BtuCD mutant in complex with BtuF, where the BtuC subunits adopt a distinct asymmetric conformation. The structure suggests that BtuF does not discriminate between, or impose, asymmetric conformations of BtuCD. It also explains the conformational disorder observed in BtuCDF crystals.


===Crystal structure of E159Q mutant of BtuCDF===
Asymmetric states of vitamin B12 transporter BtuCD are not discriminated by its cognate substrate binding protein BtuF.,Korkhov VM, Mireku SA, Hvorup RN, Locher KP FEBS Lett. 2012 Apr 5;586(7):972-6. Epub 2012 Mar 8. PMID:22569249<ref>PMID:22569249</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<!--
</div>
The line below this paragraph, {{ABSTRACT_PUBMED_22569249}}, adds the Publication Abstract to the page
<div class="pdbe-citations 4dbl" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 22569249 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_22569249}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Escherichia coli K-12]]
[[4dbl]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DBL OCA].
[[Category: Large Structures]]
 
[[Category: Hvorup RN]]
==Reference==
[[Category: Korkhov VM]]
<ref group="xtra">PMID:022569249</ref><references group="xtra"/>
[[Category: Locher KP]]
[[Category: Escherichia coli]]
[[Category: Mireku SM]]
[[Category: Vitamin B12-transporting ATPase]]
[[Category: Hvorup, R N.]]
[[Category: Korkhov, V M.]]
[[Category: Locher, K P.]]
[[Category: Mireku, S M.]]
[[Category: Abc transporter for vitamin b12]]
[[Category: Atp binding]]
[[Category: Inner membrane]]
[[Category: Transport protein]]

Latest revision as of 09:55, 30 October 2024

Crystal structure of E159Q mutant of BtuCDF

4dbl, resolution 3.49Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=4dbl&oldid=4249585"