3zqd: Difference between revisions

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[[Image:3zqd.jpg|left|200px]]


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==B. subtilis L,D-transpeptidase==
The line below this paragraph, containing "STRUCTURE_3zqd", creates the "Structure Box" on the page.
<StructureSection load='3zqd' size='340' side='right'caption='[[3zqd]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3zqd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZQD FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zqd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqd OCA], [https://pdbe.org/3zqd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zqd RCSB], [https://www.ebi.ac.uk/pdbsum/3zqd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zqd ProSAT]</span></td></tr>
{{STRUCTURE_3zqd|  PDB=3zqd  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/YKUD_BACSU YKUD_BACSU] Probable enzyme that may play an important role in cell wall biology.<ref>PMID:16287140</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
beta-lactams inhibit peptidoglycan polymerization by acting as suicide substrates of essential d,d-transpeptidases. Bypass of these enzymes by unrelated l,d-transpeptidases results in beta-lactam resistance, although carbapenems remain unexpectedly active. To gain insight into carbapenem specificity of l,d-transpeptidases (Ldts), we solved the nuclear magnetic resonance (NMR) structures of apo and imipenem-acylated Bacillus subtilis Ldt and show that the cysteine nucleophile is present as a neutral imidazole-sulfhydryl pair in the substrate-free enzyme. NMR relaxation dispersion does not reveal any preexisting conformational exchange in the apoenzyme, and change in flexibility is not observed upon noncovalent binding of beta-lactams (K(D) &gt; 37.5 mM). In contrast, covalent modification of active cysteine by both carbapenems and 2-nitro-5-thiobenzoate induces backbone flexibility that does not result from disruption of the imidazole-sulfhydryl proton interaction or steric hindrance. The chemical step of the reaction determines enzyme specificity since no differences in drug affinity were observed.


===B. subtilis L,D-transpeptidase===
Dynamics Induced by beta-Lactam Antibiotics in the Active Site of Bacillus subtilisl,d-Transpeptidase.,Lecoq L, Bougault C, Hugonnet JE, Veckerle C, Pessey O, Arthur M, Simorre JP Structure. 2012 May 9;20(5):850-61. PMID:22579252<ref>PMID:22579252</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_22579252}}, adds the Publication Abstract to the page
<div class="pdbe-citations 3zqd" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 22579252 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_22579252}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[3zqd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQD OCA].
[[Category: Large Structures]]
 
[[Category: Arthur M]]
==Reference==
[[Category: Bougault C]]
<ref group="xtra">PMID:022579252</ref><ref group="xtra">PMID:016287140</ref><references group="xtra"/>
[[Category: Hugonnet J-E]]
[[Category: Bacillus subtilis subsp. subtilis]]
[[Category: Lecoq L]]
[[Category: Arthur, M.]]
[[Category: Pessey O]]
[[Category: Bougault, C.]]
[[Category: Simorre J-P]]
[[Category: Hugonnet, J E.]]
[[Category: Veckerle C]]
[[Category: Lecoq, L.]]
[[Category: Pessey, O.]]
[[Category: Simorre, J P.]]
[[Category: Veckerle, C.]]
[[Category: Antibiotic resistance]]
[[Category: Cysteine protease]]
[[Category: Peptidoglycan]]
[[Category: Transferase]]
Retrieved from "https://proteopedia.org/3zqd"