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[[Image:1wlj.jpg|left|200px]]<br /><applet load="1wlj" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1wlj, resolution 1.9&Aring;" />
'''human ISG20'''<br />


==Overview==
==human ISG20==
ISG20 is an interferon-induced antiviral exoribonuclease that acts on, single-stranded RNA and also has minor activity towards single-stranded, DNA. It belongs to the DEDDh group of RNases of the DEDD exonuclease, superfamily. We have solved the crystal structure of human ISG20 complexed, with two Mn2+ ions and uridine 5'-monophosphate (UMP) at 1.9 A resolution., Its structure, including that of the active site, is very similar to those, of the corresponding domains of two DEDDh-group DNases, the epsilon, subunit of Escherichia coli DNA polymerase III and E. coli exonuclease I, strongly suggesting that its catalytic mechanism is identical to that of, the two DNases. However, ISG20 also has distinctive residues, Met14 and, Arg53, to accommodate hydrogen bonds with the 2'-OH group of the UMP, ribose, and these residues may be responsible for the preference of ISG20, for RNA substrates.
<StructureSection load='1wlj' size='340' side='right'caption='[[1wlj]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1wlj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WLJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WLJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wlj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wlj OCA], [https://pdbe.org/1wlj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wlj RCSB], [https://www.ebi.ac.uk/pdbsum/1wlj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wlj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ISG20_HUMAN ISG20_HUMAN] Interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. Exhibits antiviral activity against RNA viruses including hepatitis C virus (HCV), hepatitis A virus (HAV) and yellow fever virus (YFV) in an exonuclease-dependent manner. May also play additional roles in the maturation of snRNAs and rRNAs, and in ribosome biogenesis.<ref>PMID:11401564</ref> <ref>PMID:12594219</ref> <ref>PMID:16033969</ref> <ref>PMID:21036379</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wl/1wlj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wlj ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1WLJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=U5P:'>U5P</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WLJ OCA].
*[[Potassium channel 3D structures|Potassium channel 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structure of human ISG20, an interferon-induced antiviral ribonuclease., Horio T, Murai M, Inoue T, Hamasaki T, Tanaka T, Ohgi T, FEBS Lett. 2004 Nov 5;577(1-2):111-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15527770 15527770]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Hamasaki, T.]]
[[Category: Hamasaki T]]
[[Category: Horio, T.]]
[[Category: Horio T]]
[[Category: Inoue, T.]]
[[Category: Inoue T]]
[[Category: Murai, M.]]
[[Category: Murai M]]
[[Category: Ohgi, T.]]
[[Category: Ohgi T]]
[[Category: Tanaka, T.]]
[[Category: Tanaka T]]
[[Category: ACT]]
[[Category: MN]]
[[Category: U5P]]
[[Category: exoribonuclease]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:07:41 2008''

Latest revision as of 13:32, 13 March 2024

human ISG20

1wlj, resolution 1.90Å

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