1a5o: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(21 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1a5o.gif|left|200px]]<br />
<applet load="1a5o" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1a5o, resolution 2.5&Aring;" />
'''K217C VARIANT OF KLEBSIELLA AEROGENES UREASE, CHEMICALLY RESCUED BY FORMATE AND NICKEL'''<br />


==Overview==
==K217C VARIANT OF KLEBSIELLA AEROGENES UREASE, CHEMICALLY RESCUED BY FORMATE AND NICKEL==
Klebsiella aerogenes urease possesses a dinuclear metallocenter in which, two nickel atoms are bridged by carbamylated Lys217. To assess whether, carbamate-specific chemistry is required for urease activity, site-directed mutagenesis and chemical rescue strategies were combined in, efforts to place a carboxylate group at the location of this metal ligand., Urease variants with Lys217 replaced by Glu, Cys, and Ala (K217E, K217C/C319A, and K217A proteins) were purified, shown to be activated by, incubation with small organic acids plus Ni(II), and structurally, characterized. K217C/C319A urease possessed a second change in which, Cys319 was replaced by Ala in order to facilitate efforts to chemically, modify Cys217; however, this covalent modification approach did not, produce active ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9558361 (full description)]]
<StructureSection load='1a5o' size='340' side='right'caption='[[1a5o]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1a5o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A5O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A5O FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a5o OCA], [https://pdbe.org/1a5o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a5o RCSB], [https://www.ebi.ac.uk/pdbsum/1a5o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a5o ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/URE3_KLEAE URE3_KLEAE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/1a5o_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a5o ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1A5O is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]] with NI and FMT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Urease Urease]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5]]. Structure known Active Sites: ACT and NIL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A5O OCA]].
*[[Urease 3D structures|Urease 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Chemical rescue of Klebsiella aerogenes urease variants lacking the carbamylated-lysine nickel ligand., Pearson MA, Schaller RA, Michel LO, Karplus PA, Hausinger RP, Biochemistry. 1998 Apr 28;37(17):6214-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9558361 9558361]
[[Category: Klebsiella aerogenes]]
[[Category: Klebsiella aerogenes]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Urease]]
[[Category: Hausinger RP]]
[[Category: Hausinger, R.P.]]
[[Category: Karplus PA]]
[[Category: Karplus, P.A.]]
[[Category: Michel LO]]
[[Category: Michel, L.O.]]
[[Category: Pearson MA]]
[[Category: Pearson, M.A.]]
[[Category: Schaller RA]]
[[Category: Schaller, R.A.]]
[[Category: FMT]]
[[Category: NI]]
[[Category: hydrolase (urea amido)]]
[[Category: mutant]]
[[Category: nickel metalloenzyme]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:19:21 2007''
Retrieved from "https://proteopedia.org/1a5o"