3svl: Difference between revisions

Latest revision as of 13:08, 14 March 2024

Structural basis of the improvement of ChrR - a multi-purpose enzyme

Structural highlights

3svl is a 2 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:	CA, FMN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CHRR_ECOLI Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr(6+) producing Cr(3+) and one electron to molecular oxygen without producing the toxic Cr(5+) species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V(5+), Mo(6+), methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.^[1] ^[2] ^[3] ^[4]

References

↑ Ackerley DF, Gonzalez CF, Park CH, Blake R 2nd, Keyhan M, Matin A. Chromate-reducing properties of soluble flavoproteins from Pseudomonas putida and Escherichia coli. Appl Environ Microbiol. 2004 Feb;70(2):873-82. PMID:14766567
↑ Ackerley DF, Barak Y, Lynch SV, Curtin J, Matin A. Effect of chromate stress on Escherichia coli K-12. J Bacteriol. 2006 May;188(9):3371-81. PMID:16621832 doi:https://dx.doi.org/10.1128/JB.188.9.3371-3381.2006
↑ Barak Y, Ackerley DF, Dodge CJ, Banwari L, Alex C, Francis AJ, Matin A. Analysis of novel soluble chromate and uranyl reductases and generation of an improved enzyme by directed evolution. Appl Environ Microbiol. 2006 Nov;72(11):7074-82. PMID:17088379 doi:https://dx.doi.org/72/11/7074
↑ Eswaramoorthy S, Poulain S, Hienerwadel R, Bremond N, Sylvester MD, Zhang YB, Berthomieu C, Van Der Lelie D, Matin A. Crystal Structure of ChrR-A Quinone Reductase with the Capacity to Reduce Chromate. PLoS One. 2012;7(4):e36017. Epub 2012 Apr 27. PMID:22558308 doi:10.1371/journal.pone.0036017

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OCA

[1] Ackerley DF, Gonzalez CF, Park CH, Blake R 2nd, Keyhan M, Matin A. Chromate-reducing properties of soluble flavoproteins from Pseudomonas putida and Escherichia coli. Appl Environ Microbiol. 2004 Feb;70(2):873-82. PMID:14766567

[2] Ackerley DF, Barak Y, Lynch SV, Curtin J, Matin A. Effect of chromate stress on Escherichia coli K-12. J Bacteriol. 2006 May;188(9):3371-81. PMID:16621832 doi:https://dx.doi.org/10.1128/JB.188.9.3371-3381.2006

[3] Barak Y, Ackerley DF, Dodge CJ, Banwari L, Alex C, Francis AJ, Matin A. Analysis of novel soluble chromate and uranyl reductases and generation of an improved enzyme by directed evolution. Appl Environ Microbiol. 2006 Nov;72(11):7074-82. PMID:17088379 doi:https://dx.doi.org/72/11/7074

[4] Eswaramoorthy S, Poulain S, Hienerwadel R, Bremond N, Sylvester MD, Zhang YB, Berthomieu C, Van Der Lelie D, Matin A. Crystal Structure of ChrR-A Quinone Reductase with the Capacity to Reduce Chromate. PLoS One. 2012;7(4):e36017. Epub 2012 Apr 27. PMID:22558308 doi:10.1371/journal.pone.0036017

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[2]

[3]

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@@ Line 1: / Line 1: @@
-[[Image:3svl.jpg|left|200px]]
-<!--
+==Structural basis of the improvement of ChrR - a multi-purpose enzyme==
-The line below this paragraph, containing "STRUCTURE_3svl", creates the "Structure Box" on the page.
+<StructureSection load='3svl' size='340' side='right'caption='[[3svl]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3svl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SVL FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-{{STRUCTURE_3svl|  PDB=3svl  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3svl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3svl OCA], [https://pdbe.org/3svl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3svl RCSB], [https://www.ebi.ac.uk/pdbsum/3svl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3svl ProSAT]</span></td></tr>
+</table>
-===Structural basis of the improvement of ChrR - a multi-purpose enzyme===
+== Function ==
+[https://www.uniprot.org/uniprot/CHRR_ECOLI CHRR_ECOLI] Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr(6+) producing Cr(3+) and one electron to molecular oxygen without producing the toxic Cr(5+) species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V(5+), Mo(6+), methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.<ref>PMID:14766567</ref> <ref>PMID:16621832</ref> <ref>PMID:17088379</ref> <ref>PMID:22558308</ref>
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_22558308}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 22558308 is the PubMed ID number.
+</StructureSection>
--->
+[[Category: Escherichia coli K-12]]
-{{ABSTRACT_PUBMED_22558308}}
+[[Category: Large Structures]]
+[[Category: Berthomieu C]]
-==About this Structure==
+[[Category: Bremond N]]
-[[3svl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SVL OCA].
+[[Category: Eswaramoorthy S]]
+[[Category: Hienerwadel R]]
-==Reference==
+[[Category: Matin AC]]
-<ref group="xtra">PMID:022558308</ref><references group="xtra"/>
+[[Category: Poulain S]]
-[[Category: Escherichia coli]]
+[[Category: Sylvester MD]]
-[[Category: Berthomieu, C.]]
+[[Category: Van Der Lelie D]]
-[[Category: Bremond, N.]]
+[[Category: Zhang YB]]
-[[Category: Eswaramoorthy, S.]]
-[[Category: Hienerwadel, R.]]
-[[Category: Lelie, D Van Der.]]
-[[Category: Matin, A C.]]
-[[Category: Poulain, S.]]
-[[Category: Sylvester, M D.]]
-[[Category: Zhang, Y B.]]
-[[Category: Chromate bioremediation]]
-[[Category: E. coli chrr enzyme]]
-[[Category: Improved mutant enzyme]]
-[[Category: Oxidoreductase]]
-[[Category: Tetramer role]]

3svl: Difference between revisions

Latest revision as of 13:08, 14 March 2024

Structural basis of the improvement of ChrR - a multi-purpose enzyme

Structural highlights

Function

References

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