1mz5: Difference between revisions

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[[Image:1mz5.png|left|200px]]


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==Trypanosoma rangeli sialidase==
The line below this paragraph, containing "STRUCTURE_1mz5", creates the "Structure Box" on the page.
<StructureSection load='1mz5' size='340' side='right'caption='[[1mz5]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1mz5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_rangeli Trypanosoma rangeli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MZ5 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
{{STRUCTURE_1mz5|  PDB=1mz5  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mz5 OCA], [https://pdbe.org/1mz5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mz5 RCSB], [https://www.ebi.ac.uk/pdbsum/1mz5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mz5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O44049_TRYRA O44049_TRYRA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mz/1mz5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mz5 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The intracellular parasite Trypanosoma cruzi, the etiological agent of Chagas disease, sheds a developmentally regulated surface trans-sialidase, which is involved in key aspects of parasite-host cell interactions. Although it shares a common active site architecture with bacterial neuraminidases, the T.cruzi enzyme behaves as a highly efficient sialyltransferase. Here we report the crystal structure of the closely related Trypanosoma rangeli sialidase and its complex with inhibitor. The enzyme folds into two distinct domains: a catalytic beta-propeller fold tightly associated with a lectin-like domain. Comparison with the modeled structure of T.cruzi trans-sialidase and mutagenesis experiments allowed the identification of amino acid substitutions within the active site cleft that modulate sialyltransferase activity and suggest the presence of a distinct binding site for the acceptor carbohydrate. The structures of the Trypanosoma enzymes illustrate how a glycosidase scaffold can achieve efficient glycosyltransferase activity and provide a framework for structure-based drug design.


===Trypanosoma rangeli sialidase===
Structural basis of sialyltransferase activity in trypanosomal sialidases.,Buschiazzo A, Tavares GA, Campetella O, Spinelli S, Cremona ML, Paris G, Amaya MF, Frasch AC, Alzari PM EMBO J. 2000 Jan 4;19(1):16-24. PMID:10619840<ref>PMID:10619840</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1mz5" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 10619840 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_10619840}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[1mz5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_rangeli Trypanosoma rangeli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZ5 OCA].
 
==Reference==
<ref group="xtra">PMID:010619840</ref><ref group="xtra">PMID:016498413</ref><references group="xtra"/>
[[Category: Exo-alpha-sialidase]]
[[Category: Trypanosoma rangeli]]
[[Category: Trypanosoma rangeli]]
[[Category: Alzari, P M.]]
[[Category: Alzari PM]]
[[Category: Amaya, M F.]]
[[Category: Amaya MF]]
[[Category: Buschiazzo, A.]]
[[Category: Buschiazzo A]]
[[Category: Campetella, O.]]
[[Category: Campetella O]]
[[Category: Cremona, M L.]]
[[Category: Cremona ML]]
[[Category: Frasch, A C.C.]]
[[Category: Frasch ACC]]
[[Category: Paris, G.]]
[[Category: Paris G]]
[[Category: Spinelli, S.]]
[[Category: Spinelli S]]
[[Category: Tavares, G A.]]
[[Category: Tavares GA]]
[[Category: Hydrolase]]
[[Category: Inibitor complex]]
[[Category: Sialyltransferase]]
[[Category: Trypanosomal sialidase]]
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