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[[Image:1ema.png|left|200px]]


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==GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA==
The line below this paragraph, containing "STRUCTURE_1ema", creates the "Structure Box" on the page.
<StructureSection load='1ema' size='340' side='right'caption='[[1ema]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1ema]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. The June 2003 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Green Fluorescent Protein (GFP)''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2003_6 10.2210/rcsb_pdb/mom_2003_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EMA FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
{{STRUCTURE_1ema|  PDB=1ema  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ema FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ema OCA], [https://pdbe.org/1ema PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ema RCSB], [https://www.ebi.ac.uk/pdbsum/1ema PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ema ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/em/1ema_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ema ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria has generated intense interest as a marker for gene expression and localization of gene products. The chromophore, resulting from the spontaneous cyclization and oxidation of the sequence -Ser65 (or Thr65)-Tyr66-Gly67-, requires the native protein fold for both formation and fluorescence emission. The structure of Thr65 GFP has been determined at 1.9 angstrom resolution. The protein fold consists of an 11-stranded beta barrel with a coaxial helix, with the chromophore forming from the central helix. Directed mutagenesis of one residue adjacent to the chromophore, Thr203, to Tyr or His results in significantly red-shifted excitation and emission maxima.


===GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA===
Crystal structure of the Aequorea victoria green fluorescent protein.,Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075<ref>PMID:8703075</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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{{ABSTRACT_PUBMED_8703075}}
 
==About this Structure==
[[1ema]] is a 1 chain structure of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]] with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. The June 2003 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Green Fluorescent Protein (GFP)''  by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2003_6 10.2210/rcsb_pdb/mom_2003_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMA OCA].


==See Also==
==See Also==
*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
*[[GFP (Hebrew)|GFP (Hebrew)]]
*[[Gfp vc2|Gfp vc2]]
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
*[[Green Fluorescent Protein: Research Tool|Green Fluorescent Protein: Research Tool]]
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
*[[Sandbox104|Sandbox104]]
== References ==
*[[User:Amir Mitchell/hebrew GFP|User:Amir Mitchell/hebrew GFP]]
<references/>
*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
__TOC__
*[[User:Lynmarie K Thompson/2009Chem490|User:Lynmarie K Thompson/2009Chem490]]
</StructureSection>
*[[User:Pat McQuaid/Class Project|User:Pat McQuaid/Class Project]]
*[[User:Student|User:Student]]
*[[User:Wayne Decatur/Applet side by side demo|User:Wayne Decatur/Applet side by side demo]]
 
==Reference==
<ref group="xtra">PMID:008703075</ref><references group="xtra"/>
[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Ormo, M.]]
[[Category: Ormo M]]
[[Category: Remington, S J.]]
[[Category: Remington SJ]]
[[Category: Autocatalytic]]
[[Category: Beta-barrel]]
[[Category: Bioluminescense fluorescent protein]]
[[Category: Fluorescent protein]]
[[Category: Fluorophore]]

Latest revision as of 07:21, 23 October 2024

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA

1ema, resolution 1.90Å

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