Hyaluronidase: Difference between revisions

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{{STRUCTURE_1c82|  PDB=1c82  | SIZE=400| SCENE= |right|CAPTION=Hyaluronidase complex with hyaluronic acid disaccharide and dimethylarsinate, [[1c82]] }}
<StructureSection load="1c82" size="450" color="" frame="true" spin="on" Scene='49/497055/Cv/1'  caption='Hyaluronidase complex with hyaluronic acid disaccharide and dimethylarsinate, [[1c82]]' >
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== Function ==
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'''Hyaluronidase''' or '''hyaluronate lyase''' (HU) hydrolyzes complex carbohydrates such as hyaluronan which is part of the extracellular matrix.  The hydrolysis increases tissue permeability<ref>PMID:17503783</ref>.   
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'''Hyaluronidase''' (HU) hydrolyzes complex carbohydrates such as hyaluronan which is part of the extracellular matrix.  The hydrolysis increases tissue permeability.  HU is used in medicine to speed drug delivery.  Hyaluronic acid (HUA) is a popular dermal filler material.


==3D structures of hyaluronidase==
[[Image:hya_xdt_100906.jpeg|left|thumb|'''Superposition of the EGF-like domain of hyaluronidase-1 (yellow) and the heparin-binding EGF-like growth factor (light blue)'''. The three disulphide bonds of the EGF domains (highlighted red) exhibit the same pattern in the primary structure and are located in similar positions in the 3D structure. |400px]]
{{Clear}}
[[Image:Hyal reaction.jpg|thumb|left|'''Hyaluronidase cleaves the β1,4-glycosidic bond of the glycosaminoglycan hyaluronan''' |400px]]
{{Clear}}
== Relevance ==
HU is used in medicine to speed drug delivery.  Hyaluronic acid (HUA) is a popular dermal filler material.
 
== Structural highlights ==
HU structure contains an <scene name='49/497055/Cv/8'>N-terminal, linker and C-terminal domains</scene>. The active site is in a cleft in the N-terminal domain.  Two HUA molecules are bound in the active site making contacts with some HU residues and multiple contacts with water molecules<ref>PMID:10843845</ref>.
 
<scene name='49/497055/Cv/9'>1st active site of HU</scene>. Water molecules are shown as red spheres.
 
<scene name='49/497055/Cv/10'>2nd active site of HU</scene>.


<scene name='49/497055/Cv/11'>Dimethylarsinate binding site</scene>.


[[1egu]], [[2brw]] – SpHU – ''Streptococcus pneumoniae'' <br />
==3D structures of hyaluronidase==
[[1n7n]], [[1n7o]], [[1n7p]] – SpHU (mutant) <br />
[[Hyaluronidase 3D structures]]
[[1fcq]], [[1fcu]] – bHU – bee<br />
[[1f1s]] – SaHU – ''Streptococcus agalactiae'' <br />
[[2c3f]], [[2dp5]], [[2yw0]], [[2yx2]], [[2wb3]], [[2wh7]] - SpyHU – ''Streptococcus pyogenes''<br />
[[2cbi]], [[2w1q]], [[2w1s]] - CpHU – ''Clostridium perfringens''<br />
[[2jnk]] - CpHU residues 1498-1628]] - NMR<br />
[[2atm]] - HU – ''Vespula vulgaris''<br />
[[2pe4]] – HU-1 – human


'''Hyaluronidase binary complex'''
</StructureSection>


[[1c82]] – SpHU + HUA disaccharide <br />
==Reference==
[[1f9g]] – SpHU + ascorbate <br />
<references/>
[[2brv]]– SpHU + malonate<br />
<br />
[[1fcv]] - bHU + HUA<br />
Created with the participation of [[User:Osnat Herzberg|Osnat Herzberg]], [[User:Eran Hodis|Eran Hodis]], [[User:Joel L. Sussman|Joel L. Sussman]], [[User:Jaime Prilusky|Jaime Prilusky]].
[[1i8q]] - SaHU + HUA disaccharide <br />
 
[[1loh]], [[1lxk]] - SpHU + glucuronic acid<br />
[[Category:Topic Page]]
[[1n7q]], [[1n7r]] - SpHU (mutant) + glucuronic acid<br />
[[1lxm]] - SaHU + glucuronic acid<br />
[[1ojm]], [[1ojp]] - SpHU + condroitin disaccharide <br />
[[1ojn]], [[1ojo]] - SpHU (mutant) + condroitin disaccharide <br />
[[1w3y]] - SpHU + palmitoyl-vitamin C <br />
[[2cbj]] - CpHU + PugNac<br />
[[2j1a]] - CpHU + galactose<br />
[[2j1e]] - CpHU + LacNac<br />
[[2j7m]], [[2w1u]], [[2wdb]] - CpHU + carbohydrates<br />
[[2j62]] - CpHU + GlcNac-statin<br />
[[2brp]] – SpHU + inbhibitor<br />
[[2j88]] - bHU + FAB<br />
[[2yvv]] - SpyHU + lactose<br />
[[3eka]] - SpyHU + ascorbate <br />
Retrieved from "https://proteopedia.org/Hyaluronidase"