2f37: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(13 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:2f37.jpg|left|200px]]<br /><applet load="2f37" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2f37, resolution 1.70&Aring;" />
'''Crystal structure of the ankyrin repeat domain of human TRPV2'''<br />


==Overview==
==Crystal structure of the ankyrin repeat domain of human TRPV2==
TRPV channels are important polymodal integrators of noxious stimuli, mediating thermosensation and nociception. An ankyrin repeat domain (ARD), which is a common protein-protein recognition domain, is conserved in the, N-terminal intracellular domain of all TRPV channels and predicted to, contain three to four ankyrin repeats. Here we report the first structure, from the TRPV channel subfamily, a 1.7 A resolution crystal structure of, the human TRPV2 ARD. Our crystal structure reveals a six ankyrin repeat, stack with multiple insertions in each repeat generating several unique, features compared with a canonical ARD. The surface typically used for, ligand recognition, the ankyrin groove, contains extended loops with an, exposed hydrophobic patch and a prominent kink resulting from a large, rotational shift of the last two repeats. The TRPV2 ARD provides the first, structural insight into a domain that coordinates nociceptive sensory, transduction and is likely to be a prototype for other TRPV channel ARDs.
<StructureSection load='2f37' size='340' side='right'caption='[[2f37]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2f37]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F37 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F37 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TRPV2, VRL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f37 OCA], [https://pdbe.org/2f37 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f37 RCSB], [https://www.ebi.ac.uk/pdbsum/2f37 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f37 ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/TRPV2_HUMAN TRPV2_HUMAN]] Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH.<ref>PMID:10201375</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f3/2f37_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f37 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
TRPV channels are important polymodal integrators of noxious stimuli mediating thermosensation and nociception. An ankyrin repeat domain (ARD), which is a common protein-protein recognition domain, is conserved in the N-terminal intracellular domain of all TRPV channels and predicted to contain three to four ankyrin repeats. Here we report the first structure from the TRPV channel subfamily, a 1.7 A resolution crystal structure of the human TRPV2 ARD. Our crystal structure reveals a six ankyrin repeat stack with multiple insertions in each repeat generating several unique features compared with a canonical ARD. The surface typically used for ligand recognition, the ankyrin groove, contains extended loops with an exposed hydrophobic patch and a prominent kink resulting from a large rotational shift of the last two repeats. The TRPV2 ARD provides the first structural insight into a domain that coordinates nociceptive sensory transduction and is likely to be a prototype for other TRPV channel ARDs.


==About this Structure==
Crystal structure of the human TRPV2 channel ankyrin repeat domain.,McCleverty CJ, Koesema E, Patapoutian A, Lesley SA, Kreusch A Protein Sci. 2006 Sep;15(9):2201-6. Epub 2006 Aug 1. PMID:16882997<ref>PMID:16882997</ref>
2F37 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F37 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of the human TRPV2 channel ankyrin repeat domain., McCleverty CJ, Koesema E, Patapoutian A, Lesley SA, Kreusch A, Protein Sci. 2006 Sep;15(9):2201-6. Epub 2006 Aug 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16882997 16882997]
</div>
[[Category: Homo sapiens]]
<div class="pdbe-citations 2f37" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
[[Category: McCleverty, C.J.]]
<references/>
[[Category: SO4]]
__TOC__
[[Category: ankyrin repeat]]
</StructureSection>
 
[[Category: Human]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:24:40 2008''
[[Category: Large Structures]]
[[Category: McCleverty, C J]]
[[Category: Ankyrin repeat]]
[[Category: Membrane protein]]

Latest revision as of 10:37, 17 February 2021

Crystal structure of the ankyrin repeat domain of human TRPV2

2f37, resolution 1.70Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2f37&oldid=3357751"