4ewv: Difference between revisions

Latest revision as of 15:10, 14 March 2024

Crystal structure of GH3.12 in complex with AMPCPP

Structural highlights

4ewv is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.897Å
Ligands:	APC, MN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GH312_ARATH Catalyzes the conjugation of specific amino acids (e.g. Glu and possibly His, Lys, and Met) to their preferred acyl substrates (e.g. 4-substituted benzoates), in a magnesium ion- and ATP-dependent manner. Can use 4-substituted benzoates such as 4-aminobenzoate (pABA), 4-fluorobenzoate and 4-hydroxybenzoate (4-HBA), and, to a lesser extent, benzoate, vanillate and trans-cinnamate, but not 2-substituted benzoates and salicylic acid (SA), as conjugating acyl substrates. Involved in both basal and induced resistance in a SA-dependent manner. Confers resistance to virulent and avirulent pathogens (at least bacteria and oomycetes), and promotes SA glucosides accumulation. Required for the establishment of hyper-sensitive response (HR) upon incompatible interaction and subsequent systemic acquired resistance (SAR).^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7] ^[8]

References

↑ Lee MW, Jelenska J, Greenberg JT. Arabidopsis proteins important for modulating defense responses to Pseudomonas syringae that secrete HopW1-1. Plant J. 2008 May;54(3):452-65. doi: 10.1111/j.1365-313X.2008.03439.x. Epub 2008 , Feb 7. PMID:18266921 doi:10.1111/j.1365-313X.2008.03439.x
↑ Warren RF, Merritt PM, Holub E, Innes RW. Identification of three putative signal transduction genes involved in R gene-specified disease resistance in Arabidopsis. Genetics. 1999 May;152(1):401-12. PMID:10224270
↑ van der Biezen EA, Freddie CT, Kahn K, Parker JE, Jones JD. Arabidopsis RPP4 is a member of the RPP5 multigene family of TIR-NB-LRR genes and confers downy mildew resistance through multiple signalling components. Plant J. 2002 Feb;29(4):439-51. PMID:11846877
↑ McDowell JM, Williams SG, Funderburg NT, Eulgem T, Dangl JL. Genetic analysis of developmentally regulated resistance to downy mildew (Hyaloperonospora parasitica) in Arabidopsis thaliana. Mol Plant Microbe Interact. 2005 Nov;18(11):1226-34. PMID:16353557 doi:10.1094/MPMI-18-1226
↑ Lee MW, Lu H, Jung HW, Greenberg JT. A key role for the Arabidopsis WIN3 protein in disease resistance triggered by Pseudomonas syringae that secrete AvrRpt2. Mol Plant Microbe Interact. 2007 Oct;20(10):1192-200. PMID:17918621 doi:10.1094/MPMI-20-10-1192
↑ Jagadeeswaran G, Raina S, Acharya BR, Maqbool SB, Mosher SL, Appel HM, Schultz JC, Klessig DF, Raina R. Arabidopsis GH3-LIKE DEFENSE GENE 1 is required for accumulation of salicylic acid, activation of defense responses and resistance to Pseudomonas syringae. Plant J. 2007 Jul;51(2):234-46. Epub 2007 May 23. PMID:17521413 doi:10.1111/j.1365-313X.2007.03130.x
↑ Nobuta K, Okrent RA, Stoutemyer M, Rodibaugh N, Kempema L, Wildermuth MC, Innes RW. The GH3 acyl adenylase family member PBS3 regulates salicylic acid-dependent defense responses in Arabidopsis. Plant Physiol. 2007 Jun;144(2):1144-56. Epub 2007 Apr 27. PMID:17468220 doi:10.1104/pp.107.097691
↑ Okrent RA, Brooks MD, Wildermuth MC. Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate. J Biol Chem. 2009 Apr 10;284(15):9742-54. doi: 10.1074/jbc.M806662200. Epub 2009 , Feb 2. PMID:19189963 doi:10.1074/jbc.M806662200

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OCA

[1] Lee MW, Jelenska J, Greenberg JT. Arabidopsis proteins important for modulating defense responses to Pseudomonas syringae that secrete HopW1-1. Plant J. 2008 May;54(3):452-65. doi: 10.1111/j.1365-313X.2008.03439.x. Epub 2008 , Feb 7. PMID:18266921 doi:10.1111/j.1365-313X.2008.03439.x

[2] Warren RF, Merritt PM, Holub E, Innes RW. Identification of three putative signal transduction genes involved in R gene-specified disease resistance in Arabidopsis. Genetics. 1999 May;152(1):401-12. PMID:10224270

[3] van der Biezen EA, Freddie CT, Kahn K, Parker JE, Jones JD. Arabidopsis RPP4 is a member of the RPP5 multigene family of TIR-NB-LRR genes and confers downy mildew resistance through multiple signalling components. Plant J. 2002 Feb;29(4):439-51. PMID:11846877

[4] McDowell JM, Williams SG, Funderburg NT, Eulgem T, Dangl JL. Genetic analysis of developmentally regulated resistance to downy mildew (Hyaloperonospora parasitica) in Arabidopsis thaliana. Mol Plant Microbe Interact. 2005 Nov;18(11):1226-34. PMID:16353557 doi:10.1094/MPMI-18-1226

[5] Lee MW, Lu H, Jung HW, Greenberg JT. A key role for the Arabidopsis WIN3 protein in disease resistance triggered by Pseudomonas syringae that secrete AvrRpt2. Mol Plant Microbe Interact. 2007 Oct;20(10):1192-200. PMID:17918621 doi:10.1094/MPMI-20-10-1192

[6] Jagadeeswaran G, Raina S, Acharya BR, Maqbool SB, Mosher SL, Appel HM, Schultz JC, Klessig DF, Raina R. Arabidopsis GH3-LIKE DEFENSE GENE 1 is required for accumulation of salicylic acid, activation of defense responses and resistance to Pseudomonas syringae. Plant J. 2007 Jul;51(2):234-46. Epub 2007 May 23. PMID:17521413 doi:10.1111/j.1365-313X.2007.03130.x

[7] Nobuta K, Okrent RA, Stoutemyer M, Rodibaugh N, Kempema L, Wildermuth MC, Innes RW. The GH3 acyl adenylase family member PBS3 regulates salicylic acid-dependent defense responses in Arabidopsis. Plant Physiol. 2007 Jun;144(2):1144-56. Epub 2007 Apr 27. PMID:17468220 doi:10.1104/pp.107.097691

[8] Okrent RA, Brooks MD, Wildermuth MC. Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate. J Biol Chem. 2009 Apr 10;284(15):9742-54. doi: 10.1074/jbc.M806662200. Epub 2009 , Feb 2. PMID:19189963 doi:10.1074/jbc.M806662200

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@@ Line 1: / Line 1: @@
-[[Image:4ewv.jpg|left|200px]]
-<!--
+==Crystal structure of GH3.12 in complex with AMPCPP==
-The line below this paragraph, containing "STRUCTURE_4ewv", creates the "Structure Box" on the page.
+<StructureSection load='4ewv' size='340' side='right'caption='[[4ewv]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[4ewv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EWV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EWV FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.897&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-{{STRUCTURE_4ewv|  PDB=4ewv  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ewv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ewv OCA], [https://pdbe.org/4ewv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ewv RCSB], [https://www.ebi.ac.uk/pdbsum/4ewv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ewv ProSAT]</span></td></tr>
+</table>
-===Crystal structure of GH3.12 in complex with AMPCPP===
+== Function ==
+[https://www.uniprot.org/uniprot/GH312_ARATH GH312_ARATH] Catalyzes the conjugation of specific amino acids (e.g. Glu and possibly His, Lys, and Met) to their preferred acyl substrates (e.g. 4-substituted benzoates), in a magnesium ion- and ATP-dependent manner. Can use 4-substituted benzoates such as 4-aminobenzoate (pABA), 4-fluorobenzoate and 4-hydroxybenzoate (4-HBA), and, to a lesser extent, benzoate, vanillate and trans-cinnamate, but not 2-substituted benzoates and salicylic acid (SA), as conjugating acyl substrates. Involved in both basal and induced resistance in a SA-dependent manner. Confers resistance to virulent and avirulent pathogens (at least bacteria and oomycetes), and promotes SA glucosides accumulation. Required for the establishment of hyper-sensitive response (HR) upon incompatible interaction and subsequent systemic acquired resistance (SAR).<ref>PMID:18266921</ref> <ref>PMID:10224270</ref> <ref>PMID:11846877</ref> <ref>PMID:16353557</ref> <ref>PMID:17918621</ref> <ref>PMID:17521413</ref> <ref>PMID:17468220</ref> <ref>PMID:19189963</ref>
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_22628555}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 22628555 is the PubMed ID number.
+</StructureSection>
--->
-{{ABSTRACT_PUBMED_22628555}}
-==About this Structure==
-[[4ewv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EWV OCA].
-==Reference==
-<ref group="xtra">PMID:022628555</ref><references group="xtra"/>
 [[Category: Arabidopsis thaliana]]
-[[Category: Jez, J.]]
+[[Category: Large Structures]]
-[[Category: Nanao, M.]]
+[[Category: Jez J]]
-[[Category: Zubieta, C.]]
+[[Category: Nanao M]]
-[[Category: Adenylation]]
+[[Category: Zubieta C]]
-[[Category: Amino acid conjugation]]
-[[Category: Firefly luciferase family]]
-[[Category: Ligase]]

4ewv: Difference between revisions

Latest revision as of 15:10, 14 March 2024

Crystal structure of GH3.12 in complex with AMPCPP

Structural highlights

Function

References

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