1htt: Difference between revisions

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New page: left|200px<br /> <applet load="1htt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1htt, resolution 2.6Å" /> '''HISTIDYL-TRNA SYNTHE...
 
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[[Image:1htt.gif|left|200px]]<br />
<applet load="1htt" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1htt, resolution 2.6&Aring;" />
'''HISTIDYL-TRNA SYNTHETASE'''<br />


==Overview==
==HISTIDYL-TRNA SYNTHETASE==
The crystal structure at 2.6 A of the histidyl-tRNA synthetase from, Escherichia coli complexed with histidyl-adenylate has been determined., The enzyme is a homodimer with a molecular weight of 94 kDa and belongs to, the class II of aminoacyl-tRNA synthetases (aaRS). The asymmetric unit is, composed of two homodimers. Each monomer consists of two domains. The, N-terminal catalytic core domain contains a six-stranded antiparallel, beta-sheet sitting on two alpha-helices, which can be superposed with the, catalytic domains of yeast AspRS, and GlyRS and SerRS from Thermus, thermophilus with a root-mean-square difference on the C alpha atoms of, 1.7-1.9 A. The active sites of all four monomers are occupied by, histidyl-adenylate, which apparently forms during crystallization. The 100, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?7556055 (full description)]]
<StructureSection load='1htt' size='340' side='right'caption='[[1htt]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1htt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HTT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1htt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1htt OCA], [https://pdbe.org/1htt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1htt RCSB], [https://www.ebi.ac.uk/pdbsum/1htt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1htt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SYH_ECOLI SYH_ECOLI]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/1htt_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1htt ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1HTT is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with AMP as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.21 6.1.1.21]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HTT OCA]].
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of histidyl-tRNA synthetase from Escherichia coli complexed with histidyl-adenylate., Arnez JG, Harris DC, Mitschler A, Rees B, Francklyn CS, Moras D, EMBO J. 1995 Sep 1;14(17):4143-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7556055 7556055]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Arnez, J.G.]]
[[Category: Arnez JG]]
[[Category: Francklyn, C.S.]]
[[Category: Francklyn CS]]
[[Category: Harris, D.C.]]
[[Category: Harris DC]]
[[Category: Mitschler, A.]]
[[Category: Mitschler A]]
[[Category: Moras, D.]]
[[Category: Moras D]]
[[Category: Rees, B.]]
[[Category: Rees B]]
[[Category: AMP]]
[[Category: aminoacyl-trna synthase]]
[[Category: complex (trna synthetase/his-adenylate)]]
[[Category: ligase]]
[[Category: synthetase]]
 
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Latest revision as of 07:30, 7 February 2024

HISTIDYL-TRNA SYNTHETASE

1htt, resolution 2.60Å

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