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[[Image:2ibz.jpg|left|200px]]<br /><applet load="2ibz" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2ibz, resolution 2.3&Aring;" />
'''Yeast Cytochrome BC1 Complex with Stigmatellin'''<br />


==Overview==
==Yeast Cytochrome BC1 Complex with Stigmatellin==
We describe in detail the conformations of the inhibitor stigmatellin in, its free form and bound to the ubiquinone-reducing (Q(B)) site of the, reaction center and to the ubiquinol-oxidizing (Q(o)) site of the, cytochrome bc(1) complex. We present here the first structures of a, stereochemically correct stigmatellin in complexes with a bacterial, reaction center and the yeast cytochrome bc(1) complex. The conformations, of the inhibitor bound to the two enzymes are not the same. We focus on, the orientations of the stigmatellin side-chain relative to the chromone, head group, and on the interaction of the stigmatellin side-chain with, these membrane protein complexes. The different conformations of, stigmatellin found illustrate the structural variability of the Q sites, which are affected by the same inhibitor. The free rotation about the, chi(1) dihedral angle is an essential factor for allowing stigmatellin to, bind in both the reaction center and the cytochrome bc(1) pocket.
<StructureSection load='2ibz' size='340' side='right'caption='[[2ibz]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ibz]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IBZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IBZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=SMA:STIGMATELLIN+A'>SMA</scene>, <scene name='pdbligand=UQ6:5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL'>UQ6</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ibz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ibz OCA], [https://pdbe.org/2ibz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ibz RCSB], [https://www.ebi.ac.uk/pdbsum/2ibz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ibz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/QCR1_YEAST QCR1_YEAST] Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c. COR1 may mediate formation of the complex between cytochromes c and c1.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ib/2ibz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ibz ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We describe in detail the conformations of the inhibitor stigmatellin in its free form and bound to the ubiquinone-reducing (Q(B)) site of the reaction center and to the ubiquinol-oxidizing (Q(o)) site of the cytochrome bc(1) complex. We present here the first structures of a stereochemically correct stigmatellin in complexes with a bacterial reaction center and the yeast cytochrome bc1 complex. The conformations of the inhibitor bound to the two enzymes are not the same. We focus on the orientations of the stigmatellin side-chain relative to the chromone head group, and on the interaction of the stigmatellin side-chain with these membrane protein complexes. The different conformations of stigmatellin found illustrate the structural variability of the Q sites, which are affected by the same inhibitor. The free rotation about the chi1 dihedral angle is an essential factor for allowing stigmatellin to bind in both the reaction center and the cytochrome bc1 pocket.


==About this Structure==
A comparison of stigmatellin conformations, free and bound to the photosynthetic reaction center and the cytochrome bc1 complex.,Lancaster CR, Hunte C, Kelley J 3rd, Trumpower BL, Ditchfield R J Mol Biol. 2007 Apr 20;368(1):197-208. Epub 2007 Feb 11. PMID:17337272<ref>PMID:17337272</ref>
2IBZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=FES:'>FES</scene>, <scene name='pdbligand=UQ6:'>UQ6</scene> and <scene name='pdbligand=SMA:'>SMA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ubiquinol--cytochrome-c_reductase Ubiquinol--cytochrome-c reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.2.2 1.10.2.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IBZ OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
A Comparison of Stigmatellin Conformations, Free and Bound to the Photosynthetic Reaction Center and the Cytochrome bc(1) Complex., Lancaster CR, Hunte C, Kelley J 3rd, Trumpower BL, Ditchfield R, J Mol Biol. 2007 Feb 11;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17337272 17337272]
</div>
<div class="pdbe-citations 2ibz" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
*[[Cytochrome bc1 3D structures|Cytochrome bc1 3D structures]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Ubiquinol--cytochrome-c reductase]]
[[Category: Hunte C]]
[[Category: Hunte, C.]]
[[Category: FES]]
[[Category: HEM]]
[[Category: SMA]]
[[Category: UQ6]]
[[Category: multisubunit membrane protein complex]]
 
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Retrieved from "https://proteopedia.org/2ibz"