3p9y: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(5 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:3p9y.png|left|200px]]


{{STRUCTURE_3p9y| PDB=3p9y | SCENE= }}
==Crystal structure of the Drosophila melanogaster Ssu72-pCTD complex==
<StructureSection load='3p9y' size='340' side='right'caption='[[3p9y]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3p9y]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P9Y FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=N7P:1-ACETYL-L-PROLINE'>N7P</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=SET:AMINOSERINE'>SET</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9y OCA], [https://pdbe.org/3p9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p9y RCSB], [https://www.ebi.ac.uk/pdbsum/3p9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p9y ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9VWE4_DROME Q9VWE4_DROME]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
RNA polymerase II coordinates co-transcriptional events by recruiting distinct sets of nuclear factors to specific stages of transcription via changes of phosphorylation patterns along its C-terminal domain (CTD). Although it has become increasingly clear that proline isomerization also helps regulate CTD-associated processes, the molecular basis of its role is unknown. Here, we report the structure of the Ser(P)(5) CTD phosphatase Ssu72 in complex with substrate, revealing a remarkable CTD conformation with the Ser(P)(5)-Pro(6) motif in the cis configuration. We show that the cis-Ser(P)(5)-Pro(6) isomer is the minor population in solution and that Ess1-catalyzed cis-trans-proline isomerization facilitates rapid dephosphorylation by Ssu72, providing an explanation for recently discovered in vivo connections between these enzymes and a revised model for CTD-mediated small nuclear RNA termination. This work presents the first structural evidence of a cis-proline-specific enzyme and an unexpected mechanism of isomer-based regulation of phosphorylation, with broad implications for CTD biology.


===Crystal structure of the Drosophila melanogaster Ssu72-pCTD complex===
cis-Proline-mediated Ser(P)5 Dephosphorylation by the RNA Polymerase II C-terminal Domain Phosphatase Ssu72.,Werner-Allen JW, Lee CJ, Liu P, Nicely NI, Wang S, Greenleaf AL, Zhou P J Biol Chem. 2011 Feb 18;286(7):5717-26. Epub 2010 Dec 15. PMID:21159777<ref>PMID:21159777</ref>


{{ABSTRACT_PUBMED_21159777}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 3p9y" style="background-color:#fffaf0;"></div>
[[3p9y]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9Y OCA].
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:021159777</ref><references group="xtra"/>
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
[[Category: Werner-Allen, J W.]]
[[Category: Large Structures]]
[[Category: Zhou, P.]]
[[Category: Synthetic construct]]
[[Category: Cis proline]]
[[Category: Werner-Allen JW]]
[[Category: Hydrolase]]
[[Category: Zhou P]]
[[Category: Lmw ptp-like fold]]
[[Category: Phosphatase]]
[[Category: Rna polymerase ii ctd]]
Retrieved from "https://proteopedia.org/3p9y"