3vup: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: '''Unreleased structure''' The entry 3vup is ON HOLD Authors: Mizutani, K., Tsuchiya, S., Toyoda, M., Nanbu, Y., Tominaga, K., Yuasa, K., Takahashi, N., Tsuji, A., Mikami, B. Descripti... |
No edit summary |
||
| (6 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Beta-1,4-mannanase from the common sea hare Aplysia kurodai== | |||
<StructureSection load='3vup' size='340' side='right'caption='[[3vup]], [[Resolution|resolution]] 1.05Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3vup]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_kurodai Aplysia kurodai]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VUP FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.05Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vup OCA], [https://pdbe.org/3vup PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vup RCSB], [https://www.ebi.ac.uk/pdbsum/3vup PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vup ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/E5RSM0_APLKU E5RSM0_APLKU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
beta-1,4-Mannanase (EC 3.2.1.78) catalyzes the hydrolysis of beta-1,4-glycosidic bonds within mannan, a major constituent group of the hemicelluloses. Bivalves and gastropods possess beta-1,4-mannanase and may degrade mannan in seaweed and/or phytoplankton to obtain carbon and energy using the secreted enzymes in their digestive systems. In the present study, the crystal structure of AkMan, a gastropod beta-1,4-mannanase prepared from the common sea hare Aplysia kurodai, was determined at 1.05 A resolution. This is the first report of the three-dimensional structure of a gastropod beta-1,4-mannanase. The structure was compared with bivalve beta-1,4-mannanase and the roles of residues in the catalytic cleft were investigated. No obvious binding residue was found in subsite +1 and the substrate-binding site was exposed to the molecular surface, which may account for the enzymatic properties of mannanases that can digest complex substrates such as glucomannan and branched mannan. | |||
Structure of beta-1,4-mannanase from the common sea hare Aplysia kurodai at 1.05 A resolution.,Mizutani K, Tsuchiya S, Toyoda M, Nanbu Y, Tominaga K, Yuasa K, Takahashi N, Tsuji A, Mikami B Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Oct 1;68(Pt 10):1164-8., doi: 10.1107/S1744309112037074. Epub 2012 Sep 25. PMID:23027740<ref>PMID:23027740</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3vup" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Aplysia kurodai]] | |||
[[Category: Large Structures]] | |||
[[Category: Mikami B]] | |||
[[Category: Mizutani K]] | |||
[[Category: Nanbu Y]] | |||
[[Category: Takahashi N]] | |||
[[Category: Tominaga K]] | |||
[[Category: Toyoda M]] | |||
[[Category: Tsuchiya S]] | |||
[[Category: Tsuji A]] | |||
[[Category: Yuasa K]] | |||
Latest revision as of 09:49, 30 October 2024
Beta-1,4-mannanase from the common sea hare Aplysia kurodai
| ||||||||||||
