111d: Difference between revisions

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[[Image:111d.jpg|left|200px]]<br /><applet load="111d" size="350" color="white" frame="true" align="right" spinBox="true"
caption="111d, resolution 2.250&Aring;" />
'''CRYSTAL STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS'''<br />


==Overview==
==CRYSTAL STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS==
The synthetic dodecanucleotide d(CGCAAATTGGCG) has been analysed by single-crystal X-ray diffraction techniques and the structure refined to R = 0.16 and 2.25 A resolution, with the location of 94 solvent molecules. The sequence crystallizes as a full turn of a B-DNA helix with ten Watson-Crick base-pairs and two adenine-guanine mispairs. The analysis clearly shows that the mismatches are of the form A(anti).G(syn). Thermal denaturation studies indicate that the stability of the duplex is strongly pH dependent, with a maximum at pH 5.0, suggesting that the base-pair is stabilized by protonation. Three different arrangements have been observed for base-pairs between guanine and adenine and it is likely that A.G mismatch conformation is strongly influenced by dipole-dipole interactions with adjacent base-pairs.
<StructureSection load='111d' size='340' side='right'caption='[[111d]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[111d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=111D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=111D FirstGlance]. <br>
111D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=111D OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=111d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=111d OCA], [https://pdbe.org/111d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=111d RCSB], [https://www.ebi.ac.uk/pdbsum/111d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=111d ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs., Brown T, Leonard GA, Booth ED, Chambers J, J Mol Biol. 1989 May 20;207(2):455-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2754734 2754734]
__TOC__
[[Category: Protein complex]]
</StructureSection>
[[Category: Booth, E D.]]
[[Category: Large Structures]]
[[Category: Brown, T.]]
[[Category: Booth ED]]
[[Category: Chambers, J.]]
[[Category: Brown T]]
[[Category: Leonard, G A.]]
[[Category: Chambers J]]
[[Category: b-dna]]
[[Category: Leonard GA]]
[[Category: double helix]]
[[Category: mismatched]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:37:34 2008''

Latest revision as of 06:24, 7 February 2024

CRYSTAL STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS

111d, resolution 2.25Å

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