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| [[Image:192d.gif|left|200px]]<br /><applet load="192d" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="192d, resolution 1.920Å" />
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| '''RECOMBINATION-LIKE STRUCTURE OF D(CCGCGG)'''<br />
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| ==Overview== | | ==RECOMBINATION-LIKE STRUCTURE OF D(CCGCGG)== |
| We have solved the single crystal X-ray structure of the synthetic DNA hexamer d(CCGCGG). The central alternating tetramer forms a Z-DNA duplex. The initial cytosine of each strand of the duplex swings out and forms a Watson-Crick base-pair with the terminal guanine of a symmetry-related molecule. Thus, two symmetry-related DNA molecules form a twin with intermolecular base-pairs at both ends. Such a twin is additionally stabilized by a sodium ion located on a dyad axis between two DNA duplexes. The total structure has recombination-like features. It also provides a model for B/Z junctions. The crystal used in this study belongs to space group C222(1) with a = 34.33 A, b = 44.04 A and c = 38.27 A. The structure was solved by molecular replacement using partial models, and refined by molecular dynamics simulated annealing and positional treatment. The refinement has been concluded with an R-factor of 18.5% for 2377 reflections with F > or = 2 sigma (F) in the resolution region 8.0 to 1.92 A. The asymmetric unit contains two strands of d(CCGCGG) and 38 water molecules.
| | <StructureSection load='192d' size='340' side='right'caption='[[192d]], [[Resolution|resolution]] 1.92Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[192d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=192D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=192D FirstGlance]. <br> |
| 192D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=192D OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92Å</td></tr> |
| | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| ==Reference==
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=192d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=192d OCA], [https://pdbe.org/192d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=192d RCSB], [https://www.ebi.ac.uk/pdbsum/192d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=192d ProSAT]</span></td></tr> |
| Recombination-like structure of d(CCGCGG)., Malinina L, Urpi L, Salas X, Huynh-Dinh T, Subirana JA, J Mol Biol. 1994 Oct 28;243(3):484-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7966274 7966274]
| | </table> |
| [[Category: Protein complex]] | | __TOC__ |
| [[Category: Huynh-Dinh, T.]] | | </StructureSection> |
| [[Category: Malinina, L.]] | | [[Category: Large Structures]] |
| [[Category: Salas, X.]] | | [[Category: Huynh-Dinh T]] |
| [[Category: Subirana, J A.]] | | [[Category: Malinina L]] |
| [[Category: Urpi, L.]] | | [[Category: Salas X]] |
| [[Category: NA]]
| | [[Category: Subirana JA]] |
| [[Category: double helix]]
| | [[Category: Urpi L]] |
| [[Category: flipped-out bases]]
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| [[Category: z-dna]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:39:10 2008''
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