1b36: Difference between revisions
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== | ==SOLUTION STRUCTURE OF THE HAIRPIN RIBOZYME LOOP B DOMAIN RNA, NMR, 10 STRUCTURES== | ||
<StructureSection load='1b36' size='340' side='right'caption='[[1b36]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1b36]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B36 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B36 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b36 OCA], [https://pdbe.org/1b36 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b36 RCSB], [https://www.ebi.ac.uk/pdbsum/1b36 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b36 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The hairpin ribozyme is a small catalytic RNA with a unique two-domain structure. Here we present the solution structure of the loop B domain of the hairpin ribozyme, which contains most of the catalytically essential nucleotides. The 38-nucleotide domain contains a 16-nucleotide internal loop that forms one of the largest non-Watson-Crick segments of base pairing thus far determined by either NMR or crystallography. Since the solution structure of the smaller loop A domain has been previously solved, an NMR structure-based model of the 22,000 Mr hairpin ribozyme-substrate open complex emerges by joining the two domain structures. Strikingly, catalytically essential functional groups for the loop B domain are concentrated within an expanded minor groove, presenting a clear docking surface for the loop A domain. | The hairpin ribozyme is a small catalytic RNA with a unique two-domain structure. Here we present the solution structure of the loop B domain of the hairpin ribozyme, which contains most of the catalytically essential nucleotides. The 38-nucleotide domain contains a 16-nucleotide internal loop that forms one of the largest non-Watson-Crick segments of base pairing thus far determined by either NMR or crystallography. Since the solution structure of the smaller loop A domain has been previously solved, an NMR structure-based model of the 22,000 Mr hairpin ribozyme-substrate open complex emerges by joining the two domain structures. Strikingly, catalytically essential functional groups for the loop B domain are concentrated within an expanded minor groove, presenting a clear docking surface for the loop A domain. | ||
Solution structure of the loop B domain from the hairpin ribozyme.,Butcher SE, Allain FH, Feigon J Nat Struct Biol. 1999 Mar;6(3):212-6. PMID:10074938<ref>PMID:10074938</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 1b36" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Ribozyme 3D structures|Ribozyme 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | [[Category: Synthetic construct]] | ||
[[Category: Butcher | [[Category: Butcher SE]] | ||
[[Category: Dieckmann | [[Category: Dieckmann T]] | ||
[[Category: Feigon | [[Category: Feigon J]] | ||
