1ba3: Difference between revisions

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[[Image:1ba3.gif|left|200px]]<br /><applet load="1ba3" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1ba3, resolution 2.2&Aring;" />
'''FIREFLY LUCIFERASE IN COMPLEX WITH BROMOFORM'''<br />


==Overview==
==FIREFLY LUCIFERASE IN COMPLEX WITH BROMOFORM==
<StructureSection load='1ba3' size='340' side='right'caption='[[1ba3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ba3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BA3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MBR:TRIBROMOMETHANE'>MBR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ba3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ba3 OCA], [https://pdbe.org/1ba3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ba3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ba3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ba3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY] Produces green light with a wavelength of 562 nm.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ba/1ba3_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ba3 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The firefly luciferase enzyme from Photinus pyralis is probably the best-characterized model system for studying anesthetic-protein interactions. It binds a diverse range of general anesthetics over a large potency range, displays a sensitivity to anesthetics that is very similar to that found in animals, and has an anesthetic sensitivity that can be modulated by one of its substrates (ATP). In this paper we describe the properties of bromoform acting as a general anesthetic (in Rana temporaria tadpoles) and as an inhibitor of the firefly luciferase enzyme at high and low ATP concentrations. In addition, we describe the crystal structure of the low-ATP form of the luciferase enzyme in the presence of bromoform at 2.2-A resolution. These results provide a structural basis for understanding the anesthetic inhibition of the enzyme, as well as an explanation for the ATP modulation of its anesthetic sensitivity.
The firefly luciferase enzyme from Photinus pyralis is probably the best-characterized model system for studying anesthetic-protein interactions. It binds a diverse range of general anesthetics over a large potency range, displays a sensitivity to anesthetics that is very similar to that found in animals, and has an anesthetic sensitivity that can be modulated by one of its substrates (ATP). In this paper we describe the properties of bromoform acting as a general anesthetic (in Rana temporaria tadpoles) and as an inhibitor of the firefly luciferase enzyme at high and low ATP concentrations. In addition, we describe the crystal structure of the low-ATP form of the luciferase enzyme in the presence of bromoform at 2.2-A resolution. These results provide a structural basis for understanding the anesthetic inhibition of the enzyme, as well as an explanation for the ATP modulation of its anesthetic sensitivity.


==About this Structure==
Structural basis for the inhibition of firefly luciferase by a general anesthetic.,Franks NP, Jenkins A, Conti E, Lieb WR, Brick P Biophys J. 1998 Nov;75(5):2205-11. PMID:9788915<ref>PMID:9788915</ref>
1BA3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis] with <scene name='pdbligand=MBR:'>MBR</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BA3 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structural basis for the inhibition of firefly luciferase by a general anesthetic., Franks NP, Jenkins A, Conti E, Lieb WR, Brick P, Biophys J. 1998 Nov;75(5):2205-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9788915 9788915]
</div>
<div class="pdbe-citations 1ba3" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Luciferase 3D structures|Luciferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Photinus pyralis]]
[[Category: Photinus pyralis]]
[[Category: Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)]]
[[Category: Brick P]]
[[Category: Single protein]]
[[Category: Conti E]]
[[Category: Brick, P.]]
[[Category: Franks NP]]
[[Category: Conti, E.]]
[[Category: Jenkins A]]
[[Category: Franks, N P.]]
[[Category: Lieb WR]]
[[Category: Jenkins, A.]]
[[Category: Lieb, W R.]]
[[Category: MBR]]
[[Category: luminescence]]
[[Category: monooxygenase]]
[[Category: oxidoreductase]]
[[Category: photoprotein]]
 
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