3e2e: Difference between revisions

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[[Image:3e2e.png|left|200px]]


{{STRUCTURE_3e2e|  PDB=3e2e  |  SCENE=  }}
==Crystal Structure of an Intermediate Complex of T7 RNAP and 7nt of RNA==
 
<StructureSection load='3e2e' size='340' side='right'caption='[[3e2e]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
===Crystal Structure of an Intermediate Complex of T7 RNAP and 7nt of RNA===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[3e2e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E2E FirstGlance]. <br>
{{ABSTRACT_PUBMED_18948533}}
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e2e OCA], [https://pdbe.org/3e2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e2e RCSB], [https://www.ebi.ac.uk/pdbsum/3e2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e2e ProSAT]</span></td></tr>
==About this Structure==
</table>
[[3e2e]] is a 4 chain structure of [[RNA polymerase]] with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2E OCA].  
== Function ==
[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e2/3e2e_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e2e ConSurf].
<div style="clear:both"></div>


==See Also==
==See Also==
*[[Molecular Playground/T7 RNA Polymerase (7 mer int)|Molecular Playground/T7 RNA Polymerase (7 mer int)]]
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
*[[RNA polymerase|RNA polymerase]]
__TOC__
 
</StructureSection>
==Reference==
[[Category: Escherichia phage T7]]
<ref group="xtra">PMID:018948533</ref><references group="xtra"/>
[[Category: Large Structures]]
[[Category: DNA-directed RNA polymerase]]
[[Category: Synthetic construct]]
[[Category: Enterobacteria phage t7]]
[[Category: Bailey S]]
[[Category: Bailey, S.]]
[[Category: Durniak KJ]]
[[Category: Durniak, K J.]]
[[Category: Steitz TA]]
[[Category: Steitz, T A.]]
[[Category: Dna-directed rna polymerase]]
[[Category: Nucleotidyltransferase]]
[[Category: T7 rna polymerase]]
[[Category: Transcription]]
[[Category: Transferase]]
[[Category: Transferase-rna-dna complex]]
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