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| [[Image:1bam.jpg|left|200px]]<br /><applet load="1bam" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="1bam, resolution 1.95Å" />
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| '''STRUCTURE OF RESTRICTION ENDONUCLEASE BAMHI PHASED AT 1.95 ANGSTROMS RESOLUTION BY MAD ANALYSIS'''<br />
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| ==Overview== | | ==STRUCTURE OF RESTRICTION ENDONUCLEASE BAMHI PHASED AT 1.95 ANGSTROMS RESOLUTION BY MAD ANALYSIS== |
| BACKGROUND: Type II restriction endonucleases recognize DNA sequences that vary between four to eight base pairs, and require only Mg2+ as a cofactor to catalyze the hydrolysis of DNA. Their protein sequences display a surprising lack of similarity, and no recurring structural motif analogous to the helix-turn-helix or the zinc finger of transcription factors, has yet been discovered. RESULTS: We have determined the crystal structure of restriction endonuclease BamHI at 1.95 A resolution. The structure was solved by combining phase information derived from multi-wavelength X-ray data by algebraic and maximum likelihood methods. The BamHI subunit consists of a central beta-sheet with alpha-helices on both sides. The dimer configuration reveals a large cleft which could accommodate B-form DNA. Mutants of the enzyme that are deficient in cleavage are located at or near the putative DNA-binding cleft. BamHI and endonuclease EcoRI share a common core motif (CCM) consisting of five beta-strands and two helices. It remains to be determined if other restriction enzymes also contain the CCM. CONCLUSIONS: The structure of BamHI provides the first clear evidence that there may be substantial structural homology amongst restriction enzymes, even though it is undetectable at the sequence level.
| | <StructureSection load='1bam' size='340' side='right'caption='[[1bam]], [[Resolution|resolution]] 1.95Å' scene=''> |
| | == Structural highlights == |
| | <table><tr><td colspan='2'>[[1bam]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BAM FirstGlance]. <br> |
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bam OCA], [https://pdbe.org/1bam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bam RCSB], [https://www.ebi.ac.uk/pdbsum/1bam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bam ProSAT]</span></td></tr> |
| | </table> |
| | == Function == |
| | [https://www.uniprot.org/uniprot/T2BA_BACAM T2BA_BACAM] Recognizes the double-stranded sequence GGATCC and cleaves after G-1. |
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| ==About this Structure== | | ==See Also== |
| 1BAM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BAM OCA].
| | *[[BamHI|BamHI]] |
| | | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
| ==Reference==
| | __TOC__ |
| Structure of restriction endonuclease bamhi phased at 1.95 A resolution by MAD analysis., Newman M, Strzelecka T, Dorner LF, Schildkraut I, Aggarwal AK, Structure. 1994 May 15;2(5):439-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8081758 8081758]
| | </StructureSection> |
| [[Category: Bacillus amyloliquefaciens]] | | [[Category: Bacillus amyloliquefaciens]] |
| [[Category: Single protein]] | | [[Category: Large Structures]] |
| [[Category: Type II site-specific deoxyribonuclease]]
| | [[Category: Aggarwal AK]] |
| [[Category: Aggarwal, A K.]] | | [[Category: Newman M]] |
| [[Category: Newman, M.]] | |
| [[Category: endonuclease]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:53:27 2008''
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