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[[Image:3fto.png|left|200px]]


{{STRUCTURE_3fto| PDB=3fto | SCENE= }}
==Crystal structure of OppA in a open conformation==
<StructureSection load='3fto' size='340' side='right'caption='[[3fto]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3fto]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_cremoris Lactococcus cremoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FTO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fto OCA], [https://pdbe.org/3fto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fto RCSB], [https://www.ebi.ac.uk/pdbsum/3fto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fto ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/3fto_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fto ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lactococcus lactis is a promising host for (membrane) protein overproduction. Here, we describe a protocol for incorporation of selenomethionine (SeMet) into proteins expressed in L. lactis. Incorporation efficiencies of SeMet in the membrane protein complex OpuA (an ABC transporter) and the soluble protein OppA, both from L. lactis, were monitored by mass spectrometry. Both proteins incorporated SeMet with high efficiencies (&gt;90%), which greatly extends the usefulness of the expression host L. lactis for X-ray crystallography purposes. The crystal structure of ligand-free OppA was determined at 2.4 A resolution by a semiautomatic approach using selenium single-wavelength anomalous diffraction phasing.


===Crystal structure of OppA in a open conformation===
Selenomethionine incorporation in proteins expressed in Lactococcus lactis.,Berntsson RP, Alia Oktaviani N, Fusetti F, Thunnissen AM, Poolman B, Slotboom DJ Protein Sci. 2009 May;18(5):1121-7. PMID:19388077<ref>PMID:19388077</ref>


{{ABSTRACT_PUBMED_19388077}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 3fto" style="background-color:#fffaf0;"></div>
[[3fto]] is a 1 chain structure of [[Oligopeptide-binding protein]] with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._cremoris Lactococcus lactis subsp. cremoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FTO OCA].


==See Also==
==See Also==
*[[Oligopeptide-binding protein|Oligopeptide-binding protein]]
*[[ABC transporter 3D structures|ABC transporter 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:019388077</ref><references group="xtra"/>
__TOC__
[[Category: Lactococcus lactis subsp. cremoris]]
</StructureSection>
[[Category: Berntsson, R P.A.]]
[[Category: Lactococcus cremoris]]
[[Category: Fusetti, F.]]
[[Category: Large Structures]]
[[Category: Oktaviani, N A.]]
[[Category: Berntsson RP-A]]
[[Category: Poolman, B.]]
[[Category: Fusetti F]]
[[Category: Slotboom, D J.]]
[[Category: Oktaviani NA]]
[[Category: Thunnissen, A M.W H.]]
[[Category: Poolman B]]
[[Category: Oligo-peptide binding]]
[[Category: Slotboom D-J]]
[[Category: Peptide binding protein]]
[[Category: Thunnissen A-MWH]]
[[Category: Venus fly-trap]]
[[Category: Voluminous binding cavity]]
Retrieved from "https://proteopedia.org/3fto"