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[[Image:1bja.jpg|left|200px]]<br /><applet load="1bja" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1bja, resolution 2.19&Aring;" />
'''ACTIVATION DOMAIN OF THE PHAGE T4 TRANSCRIPTION FACTOR MOTA'''<br />


==Overview==
==ACTIVATION DOMAIN OF THE PHAGE T4 TRANSCRIPTION FACTOR MOTA==
Bacteriophage T4 encodes a transcription factor, MotA, that binds to the -30 region of middle-mode promoters and activates transcription by host RNA polymerase. We have solved the structure of the MotA activation domain to 2.2 A by X-ray crystallography, and have also determined its secondary structure by NMR. An area on the surface of the protein has a distinctive patch that is populated with acidic and hydrophobic residues. Mutations within this patch cause a defective T4 growth phenotype, arguing that the patch is important for MotA function. One of the mutant MotA activation domains was purified and analyzed by NMR, and the spectra clearly show that the domain is properly folded. The mutant full-length protein appears to bind DNA normally but is deficient in transcriptional activation. We conclude that the acidic/hydrophobic surface patch is specifically involved in transcriptional activation, which is reminiscent of eukaryotic acidic activation domains.
<StructureSection load='1bja' size='340' side='right'caption='[[1bja]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1bja]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BJA FirstGlance]. <br>
1BJA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJA OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bja OCA], [https://pdbe.org/1bja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bja RCSB], [https://www.ebi.ac.uk/pdbsum/1bja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bja ProSAT]</span></td></tr>
The activation domain of the MotA transcription factor from bacteriophage T4., Finnin MS, Cicero MP, Davies C, Porter SJ, White SW, Kreuzer KN, EMBO J. 1997 Apr 15;16(8):1992-2003. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9155025 9155025]
</table>
[[Category: Bacteriophage t4]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/MOTA_BPT4 MOTA_BPT4] Required for the transcriptional activation of middle promoters. Middle promoters are characterized by the presence of the conserved sequence [AT]3TGCTTNA (MotA box). MotA binds directly to MotA boxes.
[[Category: Cicero, M P.]]
__TOC__
[[Category: Davies, C.]]
</StructureSection>
[[Category: Finnin, M S.]]
[[Category: Escherichia virus T4]]
[[Category: Kreuzer, K N.]]
[[Category: Large Structures]]
[[Category: Porter, S J.]]
[[Category: Cicero MP]]
[[Category: White, S W.]]
[[Category: Davies C]]
[[Category: SO4]]
[[Category: Finnin MS]]
[[Category: activation domain]]
[[Category: Kreuzer KN]]
[[Category: alpha helical structure]]
[[Category: Porter SJ]]
[[Category: middle mode transcription]]
[[Category: White SW]]
[[Category: phage t4]]
[[Category: transcription regulation]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:55:53 2008''

Latest revision as of 06:36, 7 February 2024

ACTIVATION DOMAIN OF THE PHAGE T4 TRANSCRIPTION FACTOR MOTA

1bja, resolution 2.19Å

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