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| [[Image:1bvs.gif|left|200px]]<br /><applet load="1bvs" size="350" color="white" frame="true" align="right" spinBox="true" | | #REDIRECT [[7oa5]] This PDB entry is obsolete and replaced by 7oa5 |
| caption="1bvs, resolution 3.00Å" />
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| '''RUVA COMPLEXED TO A HOLLIDAY JUNCTION.'''<br />
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| ==Overview==
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| Holliday junctions occur as intermediates in homologous recombination and DNA repair. In bacteria, resolution of Holliday junctions is accomplished by the RuvABC system, consisting of a junction-specific helicase complex RuvAB, which promotes branch migration, and a junction-specific endonuclease RuvC, which nicks two strands. The crystal structure of a complex between the RuvA protein of M. leprae and a synthetic four-way junction has now been determined. Rather than binding on the open surface of a RuvA tetramer as previously suggested, the DNA is sandwiched between two RuvA tetramers, which form a closed octameric shell, stabilized by a conserved tetramer-tetramer interface. Interactions between the DNA backbone and helix-hairpin-helix motifs from both tetramers suggest a mechanism for strand separation promoted by RuvA.
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| ==About this Structure==
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| 1BVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_leprae Mycobacterium leprae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BVS OCA].
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| ==Reference==
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| Crystal structure of an octameric RuvA-Holliday junction complex., Roe SM, Barlow T, Brown T, Oram M, Keeley A, Tsaneva IR, Pearl LH, Mol Cell. 1998 Sep;2(3):361-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9774974 9774974]
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| [[Category: Mycobacterium leprae]]
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| [[Category: Single protein]]
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| [[Category: Pearl, L H.]]
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| [[Category: Roe, S M.]]
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| [[Category: branch migration]]
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| [[Category: dna repair]]
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| [[Category: holliday junction resolvase component]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:59:37 2008''
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