2vel: Difference between revisions
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==Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties== | |||
<StructureSection load='2vel' size='340' side='right'caption='[[2vel]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2vel]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VEL FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PGA:2-PHOSPHOGLYCOLIC+ACID'>PGA</scene></td></tr> | |||
== | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vel OCA], [https://pdbe.org/2vel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vel RCSB], [https://www.ebi.ac.uk/pdbsum/2vel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vel ProSAT]</span></td></tr> | ||
[[2vel]] | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/TPIS_TRYBB TPIS_TRYBB] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ve/2vel_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vel ConSurf]. | |||
<div style="clear:both"></div> | |||
==See Also== | ==See Also== | ||
*[[Triose phosphate isomerase 3D structures|Triose phosphate isomerase 3D structures]] | |||
*[[Triose | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: | |||
[[Category: Trypanosoma brucei brucei]] | [[Category: Trypanosoma brucei brucei]] | ||
[[Category: Alahuhta | [[Category: Alahuhta M]] | ||
[[Category: Augustyns | [[Category: Augustyns K]] | ||
[[Category: Casteleijn | [[Category: Casteleijn MG]] | ||
[[Category: El-Sayed | [[Category: El-Sayed I]] | ||
[[Category: Kemmer | [[Category: Kemmer C]] | ||
[[Category: Neubauer | [[Category: Neubauer P]] | ||
[[Category: Salin | [[Category: Salin M]] | ||
[[Category: Wierenga | [[Category: Wierenga RK]] | ||
Latest revision as of 07:07, 1 May 2024
Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties
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