3o6l: Difference between revisions

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[[Image:3o6l.png|left|200px]]


{{STRUCTURE_3o6l| PDB=3o6l | SCENE= }}
==Anti-Tat HIV 11H6H1 Fab' complexed with a 15-mer Tat peptide==
<StructureSection load='3o6l' size='340' side='right'caption='[[3o6l]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3o6l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O6L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O6L FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o6l OCA], [https://pdbe.org/3o6l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o6l RCSB], [https://www.ebi.ac.uk/pdbsum/3o6l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o6l ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q98XH7_9HIV1 Q98XH7_9HIV1]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Tat, the transcriptional activator protein of human immunodeficiency virus type 1 (HIV-1), is critical for viral replication and is a potential HIV-1 vaccine candidate. This intrinsically disordered protein is present in the extracellular medium and is involved in the pathogenicity of HIV through its interaction with different cellular and viral biological partners. A monoclonal antibody termed 11H6H1, which is specific for the N-terminal region of Tat, was selected for a functional and structural study of the HIV-1 Tat protein. The equilibrium dissociation constants (K(d)) of Tat and Tat fragments complexed with 11H6H1 were estimated by competitive ELISA. Tat contains a single tryptophan residue, Trp11, located in the N-terminal region. We show that the substitution of Trp11 by a phenylalanine completely abolishes the binding of 11H6H1, whereas the transactivating activity of Tat is preserved. The epitope recognized by 11H6H1 was restricted to the 9-mer peptide P(6)KLEPWKHP(14) centered on Trp11. The crystal structures of this 9-mer peptide and of an overlapping 15-mer peptide were determined in complex with Fab' 11H6H1 at 2.4 A and 2.1 A resolution, respectively. Tat is intrinsically disordered and can undergo induced folding upon association with a biological partner. Our crystallographic study reveals that the two Tat peptides, which are lodged in the U-shaped groove of the Fab' antigen-binding site, adopt a standard type I beta-turn conformation. The central Trp11 that is critical for Fab' recognition is further stabilized by pi-stacking interactions. The structural and biological consequences of this induced folding in HIV pathogenesis are discussed.


===Anti-Tat HIV 11H6H1 Fab' complexed with a 15-mer Tat peptide===
Fab'-Induced Folding of Antigenic N-Terminal Peptides from Intrinsically Disordered HIV-1 Tat Revealed by X-ray Crystallography.,Serriere J, Dugua JM, Bossus M, Verrier B, Haser R, Gouet P, Guillon C J Mol Biol. 2010 Oct 28. PMID:21035463<ref>PMID:21035463</ref>


{{ABSTRACT_PUBMED_21035463}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 3o6l" style="background-color:#fffaf0;"></div>
[[3o6l]] is a 3 chain structure of [[Antibody]] with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O6L OCA].


==See Also==
==See Also==
*[[Antibody|Antibody]]
*[[Antibody 3D structures|Antibody 3D structures]]
 
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
==Reference==
== References ==
<ref group="xtra">PMID:021035463</ref><ref group="xtra">PMID:020034112</ref><references group="xtra"/>
<references/>
__TOC__
</StructureSection>
[[Category: Human immunodeficiency virus 1]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Gouet, P.]]
[[Category: Gouet P]]
[[Category: Guillon, C.]]
[[Category: Guillon C]]
[[Category: Serriere, J.]]
[[Category: Serriere J]]
[[Category: Antigen-binding site]]
[[Category: Immune system]]
[[Category: Tat hiv]]
[[Category: U-shaped groove]]
Retrieved from "https://proteopedia.org/3o6l"